Plant Cell Reports

, Volume 27, Issue 9, pp 1541–1550

Identification of grapevine aquaporins and expression analysis in developing berries

  • Romain Fouquet
  • Céline Léon
  • Nathalie Ollat
  • François Barrieu
Physiology and Biochemistry

DOI: 10.1007/s00299-008-0566-1

Cite this article as:
Fouquet, R., Léon, C., Ollat, N. et al. Plant Cell Rep (2008) 27: 1541. doi:10.1007/s00299-008-0566-1

Abstract

Aquaporins are membrane water channels that play critical roles in controlling the water content of cells and tissues. In this work, nine full-length cDNAs encoding putative aquaporins were isolated from grape berry cDNA libraries. A phylogenetic analysis conducted with 28 aquaporin genes identified in the grapevine genome and previously characterized aquaporins from Arabidopsis indicates that three cDNAs encode putative tonoplast aquaporins (TIPs) whereas six cDNAs belong to the plasma membrane aquaporin subfamily (PIPs). Specific probes designed on the 3′ untranslated regions of each cDNA were used for the preparation of cDNA macroarray filters and in situ hybridization experiments. Macroarray data indicate that expression levels of most TIP and PIP genes depend on grape berry developmental stages and point out to a global decrease of aquaporin gene expression during berry ripening. In young berries, high expression of aquaporin genes was preferentially observed in dividing and elongating cells and in cells involved in water and solutes transport. Taken together, the data provided in this paper indicate that aquaporins are implicated in various physiological aspects of grape berry development.

Keywords

AquaporinsGrape berryGene expressionIn situ hybridizationFruit development

Supplementary material

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Romain Fouquet
    • 1
  • Céline Léon
    • 1
  • Nathalie Ollat
    • 1
  • François Barrieu
    • 1
  1. 1.Institut des Sciences de la Vigne et du Vin (ISVV), Unité Mixte de Recherche Ecophysiologie et Génomique Fonctionnelle de la Vigne, Institut National de la Recherche Agronomique, Domaine de la Grande FerradeUniversité de Bordeaux 1, Université Victor Ségalen Bordeaux 2Villenave d’OrnonFrance