Plant Cell Reports

, Volume 26, Issue 9, pp 1635–1646

Overexpression of SBPase enhances photosynthesis against high temperature stress in transgenic rice plants

  • Lingling Feng
  • Kun Wang
  • Yang Li
  • Yanping Tan
  • Jin Kong
  • Hui Li
  • Yangsheng Li
  • Yingguo Zhu
Biotic and Abiotic Stress

DOI: 10.1007/s00299-006-0299-y

Cite this article as:
Feng, L., Wang, K., Li, Y. et al. Plant Cell Rep (2007) 26: 1635. doi:10.1007/s00299-006-0299-y

Abstract

Activity of the Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase (SBPase) was increased by overexpression of a rice plants 9,311 (Oryza sativa L.) cDNA in rice plants zhonghua11 (Oryza sativa L.). The genetic engineering enabled the plants to accumulate SBPase in chloroplasts and resulted in enhanced tolerance to high temperature stress during growth of young seedlings. Moreover, CO2 assimilation of transgenic plants was significantly more tolerant to high temperature than that of wild-type plants. The analyses of chlorophyll fluorescence and the content and activation of SBPase indicated that the enhancement of photosynthesis to high temperature was not related to the function of photosystem II but to the content and activation of SBPase. Western blotting analyses showed that high temperature stress led to the association of SBPase with the thylakoid membranes from the stroma fractions. However, such an association was much more pronounced in wild-type plants than that in transgenic plants. The results in this study suggested that under high temperature stress, SBPase maintained the activation of ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) by preventing the sequestration of Rubisco activase to the thylakoid membranes from the soluble stroma fraction and thus enhanced the tolerance of CO2 assimilation to high temperature stress. The results suggested that overexpression of SBPase might be an effective method for enhancing high temperature tolerance of plants.

Keywords

Overexpression High temperature stress Photosynthesis Sedoheptulose-1,7-bisphosphatase Transgenic rice 

List of Abbreviations

PRK

Phosphoribulokinase

Rubisco

Ribulose-1,5-bisphosphate carboxylase–oxygenase

RuBP

Ribulose-1,5-bisphosphate

Ru5P

Ribulose-5-bisphosphate

SBP

Sedoheptulose-1,7-bisphosphate

SBPase

Sedoheptulose-1,7-bisphosphatase

PSII

Photosystem II

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Lingling Feng
    • 1
  • Kun Wang
    • 1
  • Yang Li
    • 1
  • Yanping Tan
    • 1
  • Jin Kong
    • 1
  • Hui Li
    • 1
  • Yangsheng Li
    • 1
  • Yingguo Zhu
    • 1
  1. 1.Key Laboratory of MOE for Plant Developmental Biology, College of Life SciencesWuhan UniversityWuhanPeople’s Republic of China