Genetic Transformation and Hybridization

Plant Cell Reports

, Volume 26, Issue 6, pp 791-804

First online:

Pyramiding transgenic resistance in elite indica rice cultivars against the sheath blight and bacterial blight

  • S. MaruthasalamAffiliated withRice Transformation Laboratory, Center for Plant Molecular Biology, Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University
  • , K. KalpanaAffiliated withRice Transformation Laboratory, Center for Plant Molecular Biology, Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University
  • , K. K. KumarAffiliated withRice Transformation Laboratory, Center for Plant Molecular Biology, Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University
  • , M. LoganathanAffiliated withRice Transformation Laboratory, Center for Plant Molecular Biology, Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University
  • , K. PoovannanAffiliated withRice Transformation Laboratory, Center for Plant Molecular Biology, Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University
  • , J. A. J. RajaAffiliated withRice Transformation Laboratory, Center for Plant Molecular Biology, Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University
  • , E. KokiladeviAffiliated withRice Transformation Laboratory, Center for Plant Molecular Biology, Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University
  • , R. SamiyappanAffiliated withCenter for Plant Protection Studies, Department of Plant Pathology, Tamil Nadu Agricultural University
  • , D. SudhakarAffiliated withRice Transformation Laboratory, Center for Plant Molecular Biology, Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University
    • , P. BalasubramanianAffiliated withRice Transformation Laboratory, Center for Plant Molecular Biology, Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University Email author 

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Abstract

Elite indica rice cultivars were cotransformed with genes expressing a rice chitinase (chi11) and a thaumatin-like protein (tlp) conferring resistance to fungal pathogens and a serine-threonine kinase (Xa21) conferring bacterial blight resistance, through particle bombardment, with a view to pyramiding sheath blight and bacterial blight resistance. Molecular analyses of putative transgenic lines by polymerase chain reaction, Southern Blot hybridization, and Western Blotting revealed stable integration and expression of the transgenes in a few independent transgenic lines. Progeny analyses showed the stable inheritance of transgenes to their progeny. Coexpression of chitinase and thaumatin-like protein in the progenies of a transgenic Pusa Basmati1 line revealed an enhanced resistance to the sheath blight pathogen, Rhizoctonia solani, as compared to that in the lines expressing the individual genes. A transgenic Pusa Basmati1 line pyramided with chi11, tlp, and Xa21 showed an enhanced resistance to both sheath blight and bacterial blight.

Keywords

Bacterial blight Chitinase Disease resistance Sheath blight Thaumatin-like protein