Occurrence of iridoid glycosides in in vitro cultures and intact plants of Scrophularia nodosa L.
- Katja SesterhennAffiliated withInstitut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg
- , Melanie DistlAffiliated withInstitut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg
- , Michael WinkAffiliated withInstitut für Pharmazie und Molekulare Biotechnologie, Universität Heidelberg Email author
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Shoot, root, and callus cultures of Scrophularia nodosa L. (Scrophulariaceae) were established and cultivated in vitro. Iridoid glycosides, such as harpagoside, aucubin, and catalpol were identified by LC-ESI-MS and their contents determined by HPLC. For comparison intact plants of S. nodosa were analysed. In shoot cultures slightly lower amounts of detectable iridoid glycosides (4.36% dry weight) were determined than in the field grown plants (4.88%). Concentration of harpagoside was highest in leaves of field plants (1.05%) and in flowers of in vitro plantlets (1.10%). For aucubin the highest amount was found in the leaves of in vitro plantlets (1.67%) whereas the levels of aucubin in the leaves of field plants were remarkably lower. Catalpol was produced as a trace compound in intact plants and shoot cultures. Callus and root cultures were apparently not able to synthesise iridoid glycosides.
KeywordsScrophularia nodosa Iridoid glycosides Harpagoside Aucubin Plant in vitro culture
- Occurrence of iridoid glycosides in in vitro cultures and intact plants of Scrophularia nodosa L.
Plant Cell Reports
Volume 26, Issue 3 , pp 365-371
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- Scrophularia nodosa
- Iridoid glycosides
- Plant in vitro culture
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