Plant Cell Reports

, Volume 25, Issue 11, pp 1226–1232

Expression of an Arabidopsis CAX2 variant in potato tubers increases calcium levels with no accumulation of manganese

  • Chang Kil Kim
  • Jeung-Sul Han
  • Hyun-Suk Lee
  • Joung-Youl Oh
  • Thoshiro Shigaki
  • Sung Hun Park
  • Kendal Hirschi
Genetic Transformation and Hybridization

DOI: 10.1007/s00299-006-0214-6

Cite this article as:
Kim, C.K., Han, JS., Lee, HS. et al. Plant Cell Rep (2006) 25: 1226. doi:10.1007/s00299-006-0214-6

Abstract

Previously, we made a chimeric Arabidopsis thaliana vacuolar transporter CAX2B [a variant of N-terminus truncated form of CAX2 (sCAX2) containing the “B” domain from CAX1] that has enhanced calcium (Ca2+) substrate specificity and lost the manganese (Mn2+) transport capability of sCAX2. Here, we demonstrate that potato (Solanum tuberosum L.) tubers expressing the CAX2B contain 50–65% more calcium (Ca2+) than wild-type tubers. Moreover, expression of CAX2B in potatoes did not show any significant increase of the four metals tested, particularly manganese (Mn2+). The CAX2B-expressing potatoes have normally undergone the tuber/plant/tuber cycle for three generations; the trait appeared stable through the successive generations and showed no deleterious alternations on plant growth and development. These results demonstrate the enhanced substrate specificity of CAX2B in potato. Therefore, CAX2B can be a valuable tool for Ca2+ nutrient enrichment of potatoes with reduced accumulation of undesirable metals.

Keywords

ArabidopsisCalciumH+/Ca2+ antiporterManganesePotato

Abbreviation

CAX

Cation exchanger

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Chang Kil Kim
    • 1
  • Jeung-Sul Han
    • 2
  • Hyun-Suk Lee
    • 3
  • Joung-Youl Oh
    • 1
  • Thoshiro Shigaki
    • 5
  • Sung Hun Park
    • 4
  • Kendal Hirschi
    • 4
    • 5
  1. 1.Department of HorticultureSangju National UniversitySangjuKorea
  2. 2.National Horticultural Research InstituteRural Development AdministrationSuwonKorea
  3. 3.Department of Horticultural ResearchGyeongbuk Agricultural Technology AdministrationDaeguKorea
  4. 4.Vegetable and Fruit Improvement CenterTexas A&M UniversityCollege StationUSA
  5. 5.Baylor College of Medicine, Departments of Pediatrics and Human and Molecular GeneticsUSDA/ARS Children’s Nutrition Research CenterHoustonUSA