Plant Cell Reports

, Volume 25, Issue 8, pp 836–847

A comprehensive expression analysis of the WRKY gene superfamily in rice plants during defense response

  • Hak-Seung Ryu
  • Muho Han
  • Sang-Kyu Lee
  • Jung-Il Cho
  • Nayeon Ryoo
  • Sunggi Heu
  • Youn-Hyung Lee
  • Seong Hee Bhoo
  • Guo-Liang Wang
  • Tae-Ryong Hahn
  • Jong-Seong Jeon
Genetics and Genomics

DOI: 10.1007/s00299-006-0138-1

Cite this article as:
Ryu, HS., Han, M., Lee, SK. et al. Plant Cell Rep (2006) 25: 836. doi:10.1007/s00299-006-0138-1

Abstract

To understand the transcriptional regulatory mechanism of host genes during the activation of defense responses in rice, we isolated WRKY transcription factors whose expressions were altered upon attack of the fungal pathogen Magnaporthe grisea, the causal agent of the devastating rice blast disease. A systematic expression analysis of OsWRKYs (Oryza sativa L. WRKYs) revealed that among 45 tested genes the expression of 15 genes was increased remarkably in an incompatible interaction between rice and M. grisea. Twelve of the M. grisea-inducible OsWRKY genes were also differentially regulated in rice plants infected with the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). In experiments with defense signaling molecules, the expression of two genes, OsWRKY45 and OsWRKY62, was increased in salicylic acid (SA)-treated leaves and the expression of three genes, OsWRKY10, OsWRKY82, and OsWRKY85 was increased by jasmonic acid (JA) treatment. OsWRKY30 and OsWRKY83 responded to both SA- and JA treatments. The expression profiles suggest that a large number of WRKY DNA-binding proteins are involved in the transcriptional activation of defense-related genes in response to rice pathogens.

Keywords

JA Magnaporthe grisea Rice SA WRKY Xanthomonas oryzae pv. oryzae 

Supplementary material

299_2006_138_MOESEM1_ESM.pdf (89 kb)
S1 Primers used to amplify gene-specific probe regions

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Hak-Seung Ryu
    • 1
  • Muho Han
    • 1
  • Sang-Kyu Lee
    • 1
  • Jung-Il Cho
    • 1
  • Nayeon Ryoo
    • 1
  • Sunggi Heu
    • 2
  • Youn-Hyung Lee
    • 1
  • Seong Hee Bhoo
    • 1
  • Guo-Liang Wang
    • 3
  • Tae-Ryong Hahn
    • 1
  • Jong-Seong Jeon
    • 1
  1. 1.Graduate School of Biotechnology & Plant Metabolism Research CenterKyung Hee UniversityYonginKorea
  2. 2.Plant Pathology DivisionNational Institute of Agricultural Science and Technology, Rural Development AdministrationSuwonKorea
  3. 3.Department of Plant PathologyThe Ohio State UniversityColumbusUSA

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