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In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill. cv. Zhanhua

  • Genetics and Genomics
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Abstract

Tetraploid plants of Zizyphus jujuba Mill. cv. Zhanhua were obtained with in vitro colchicine treatment. Shoot tips from in vitro-grown plants were treated with five different concentrations of colchicine (0.01, 0.03, 0.05, 0.1, 0.3%) in liquid MS medium (Murashige and Skoog 1962), and shaken (100 rpm) at 25 °C in darkness for 24, 48, 72 or 96 h, respectively. Tetraploids were obtained at a frequency of over 3% by using 0.05% colchicine (48 h, 72 h) and 0.1% colchicine (24 h, 48 h) treatment as determined by flow cytometry. Cytological and morphological evidence confirmed the results of flow cytometric analysis. The chromosome number of diploid plants was 24 and that of tetraploid plants was 48. The stomata sizes of tetraploid plants were significantly larger than those of diploid plants, while the frequency of stomata were reduced significantly. Similarly, the chloroplast number of guard cells of tetraploid plants increased significantly. The selected tetraploid plants were grafted onto mature trees of Z. jujuba Mill. cv. Zhanhua in the field, resulted in thicker stems, rounder and succulent leaves, larger flowers and a delay in florescence time (3–4 days later) than diploid plants.

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Abbreviations

BA :

Benzylaminopurine

GA 3 :

Gibberellic acid

IAA :

Indole-3-acetic acid

IBA :

Indole-3-butyric acid

MS :

Murashige and Skoog (1962) medium

References

  • Aryavand A, Ehdaie B, Tran B, Waines JG (2003) Stomata frequency and size differentiate ploidy levels in Aegilops neglecta. Genet Res Crop Evol 50:175–182

    Article  CAS  Google Scholar 

  • Borrino EM, Powell W (1988) Stomata guard cell length as an indicator of ploidy in microspore-derived plants of barley. Genome 30:158–160

    Article  Google Scholar 

  • Chen ZL, Yan ZL, Xue H, Feng XD, Chen GL, Cao JY (2002) In vitro culture of leaves and plantlet regeneration of Zizyphus jujuba var Zhanhua-Dongzao. Plant Physiol Commun 38:584

    Google Scholar 

  • Cohen D, Yao JL (1996) In vitro chromosome doubling of nine Zantedeschia cultivars. Plant Cell Tissue Organ Cult 47:43–49

    Article  Google Scholar 

  • Derman H (1954) Colchiploidy in grapes. J Hered 45:159–172

    Google Scholar 

  • Derman H (1965) Colchiploidy and histological imbalance in triploid apple and pear. Am J Bot 52:353–359

    Article  Google Scholar 

  • Dewey DR (1980) Some applications and misapplications of induced polyploidy to plant breeding. In: Lewis WH (ed) Polyploidy-biological relevance. Plenum Press, New York, pp 445–489

    Google Scholar 

  • Evans WD (1982) Guelph SO1 synthetic octoploid strawberry breeding clone. Hort Sci 17:833–834

    Google Scholar 

  • Gu XF, Zhang JR (2005) An efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujuba Mill.) using leaf explants. Plant Cell Rep 23:775–779

    Article  PubMed  CAS  Google Scholar 

  • Hamill SD, Smith MK, Dodd WA (1992) In vitro induction of banana autotetraploidy by colchicine treatment of micropropagated diploids. Aust J Bot 40:887–896.

    Article  CAS  Google Scholar 

  • James DJ, Mackenzie KAD, Malhotra SB (1987) The induction of hexaploidy in cherry rootstocks using in vitro regeneration techniques. Theor Appl Genet 73:589–594

    Article  Google Scholar 

  • Kadota M, Niimi Y (2002) In vitro induction of tetraploid plants from a diploid Japanese pear cultivar (Pyrus pyrifolia N. cv. Hosui). Plant Cell Rep 21:282–286

    Article  CAS  Google Scholar 

  • Li HW, Feng SQ, Zhao YM (1999) Primary study on the storage of Chinese jujube dongzao. J Shanxi Agric Sci 27:65–67

    Google Scholar 

  • Liu P, Xue HZ, Zhou XY, Liu MJ, Mao YM, Zhou JY (2004) Study on the biological basis of pollination in Chinese jujube (Zizyphus jujuba) and wild jujube (Z. spinosa). J Fruit Sci 21:224–228

    Google Scholar 

  • Liu QZ, Zhao HJ, Liu P, Mong RG, Freddi H (2001) Regeneration of tetraploid plants of royal gala apple variety from in vitro leave treated with colchicine. J Fruit Sci 18:7–10

    Google Scholar 

  • Masterson J (1994) Stomatal size in fossil plants:evidence for polyploidy in majority of angiosperms. Science 264:421–424

    Article  PubMed  Google Scholar 

  • Mathur N, Ramawat KG, Nandwani D (1995) Rapid in vitro multiplication of jujube through mature stem explants. Plant Cell Tissue Organ Cult 43:75–77

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19:389–404

    Google Scholar 

  • Perry JL, Lyrene PM (1984) In vitro induction of tetraploidy in Vaccinium darrowi, V. elliottii and V. darrowi × V. elliottii with colchicines treatment. J Am Soc Hort Sci 109:4–6

    Google Scholar 

  • Predieri S (2001) Mutation induction and tissue culture in improving fruits. Plant Cell Tissue Organ Cult 64:185–210

    Article  CAS  Google Scholar 

  • Sanford JC (1983) Ploidy manipulations. In: Moore JN, Janick J (eds) Methods in fruit breeding. Purdue University Press, West Lafayette, pp 100–123

    Google Scholar 

  • Sari N, Abak K, Pitrat M (1999) Comparison of ploidy level screening methods in watermelon: Citrullus lanatus (Thunb.) Matusum. and Nakai. Sci Hortic 82:265–277

    Article  Google Scholar 

  • Sebastiampillai AR, Jones JK (1976) Improved techniques for the induction and isolation of polyploids in the genus Fragaria. Euphytica 25:725–735

    Article  Google Scholar 

  • Van DM, Morpurgo R, Dolezel J, Afza R (1996) Induction and verification of autotetraploids in diploid banana (Musa acuminata) by in vitro techniques. Euphytica 88:25–34

    Article  Google Scholar 

  • Yang J (2001) The formation and evolution of polyploid genomes in plants. Acta Phytol Sin 39:357–371

    Google Scholar 

  • Yudanova SS, Maletskaya EI, Maletskii SI (2002) Variability of chloroplast number in populations of stomatal guard cells in sugar beet Beta vulgaris L. Russ J Genet 38:72–78

    Article  Google Scholar 

  • Zhao J, Liu MJ (2003) RAPD analysis on the outstanding clones of Zanhuadazao and Zanxindazao. Sci Silvae Sin 39:153–156

    Google Scholar 

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (30300242), the project of Department of Science and Technology of Shandong and the China Postdoctoral Science Foundation

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Authors and Affiliations

  1. School of Life Science, Shandong University, 27 Shanda Nanlu, Jinan, 250100, People's Republic of China

    X. F. Gu, A. F. Yang, H. Meng & J. R. Zhang

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  1. X. F. Gu
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  2. A. F. Yang
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  3. H. Meng
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  4. J. R. Zhang
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Correspondence to J. R. Zhang.

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Communicated by P. Ozias-Akins

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Gu, X.F., Yang, A.F., Meng, H. et al. In vitro induction of tetraploid plants from diploid Zizyphus jujuba Mill. cv. Zhanhua. Plant Cell Rep 24, 671–676 (2005). https://doi.org/10.1007/s00299-005-0017-1

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  • DOI: https://doi.org/10.1007/s00299-005-0017-1

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