Plant Cell Reports

, Volume 23, Issue 10, pp 678–682

Development of transgenic rice plants overexpressing the Arabidopsis H+/Ca2+ antiporter CAX1 gene

Authors

    • Institute of Genetic EngineeringKyungpook National University
  • Y.-H. Park
    • Department of Agronomy, College of AgricultureKyungpook National University
  • C. K. Kim
    • Department of HorticultureSangju National University
  • K. Hirschi
    • Department of Pediatrics and Human and Molecular Genetics, Childrens Nutrition Research CenterBaylor College of Medicine
  • J.-K. Sohn
    • Department of Agronomy, College of AgricultureKyungpook National University
Genetic Transformation and Hybridization

DOI: 10.1007/s00299-004-0861-4

Cite this article as:
Kim, K., Park, Y., Kim, C.K. et al. Plant Cell Rep (2005) 23: 678. doi:10.1007/s00299-004-0861-4

Abstract

The gene of the Arabidopsis thaliana H+/Ca2+ transporter, CAX1 (cation exchanger 1) was introduced into Japonica cultivars of rice (Ilpumbyeo) by Agrobacterium-mediated transformation, and a large number of transgenic plants were produced. The neomycin phosphotransferase II (NPTII) gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice callus. The introduction of the CAX1 gene was also proven by PCR using CAX1-specific oligonucleotide primers in regenerated plants. Stable integration and expression of the CAX1 gene in T0 plants and T1 progeny were confirmed by DNA hybridization, Northern blot analysis, and luminescent analysis.

Keywords

CAX1Agrobacterium-mediatedTransformationCalciumRice

Copyright information

© Springer-Verlag 2004