Plant Cell Reports

, Volume 23, Issue 9, pp 632–638

Expression of viral EPS-depolymerase reduces fire blight susceptibility in transgenic pear

  • Mickaël Malnoy
  • Mohamed Faize
  • Jean-Stéphane Venisse
  • Klaus Geider
  • Elisabeth Chevreau
Genetic Transformation and Hybridization

DOI: 10.1007/s00299-004-0855-2

Cite this article as:
Malnoy, M., Faize, M., Venisse, JS. et al. Plant Cell Rep (2005) 23: 632. doi:10.1007/s00299-004-0855-2

Abstract

Erwinia amylovora is the causal agent of fire blight of Maloideae. One of the main pathogenicity factors of this bacterium is the exopolysaccharide (EPS) of its capsule. In this paper, we used genetic transformation tools to constitutively express an EPS-depolymerase transgene in the pear (Pyrus communis L.) cv. Passe Crassane with the aim of decreasing its high susceptibility to fire blight. Expression of the depolymerase gene in 15 independent transgenic clones led, on average, to low depolymerase activity, although relatively high expression was observed at the transcriptional and translational levels. Only two of the transgenic clones (9X and 10M) consistently showed a decrease in fire blight susceptibility in vitro and in the greenhouse. These clones were also among the highest expressers of depolymerase at the RNA and enzyme activity levels. The correlation observed among all transgenic clones between depolymerase expression and fire blight resistance suggested the potential of this strategy.

Keywords

Fire blightAmylovoran lyasePearResistanceTransgenic trees

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Mickaël Malnoy
    • 1
    • 2
  • Mohamed Faize
    • 1
    • 3
  • Jean-Stéphane Venisse
    • 1
    • 4
  • Klaus Geider
    • 5
  • Elisabeth Chevreau
    • 1
  1. 1.UMR Génétique et Horticulture (INRA/INH/UA)Beaucouzé cedexFrance
  2. 2.Department of Plant Pathology, NYSAESUniversity of CornellGenevaUSA
  3. 3.National Institute for Agro-Environmental SciencesTsukubaJapan
  4. 4.UMR1095, INRA Amélioration et Santé des PlantesUniversité Blaise PascaleAubière cedexFrance
  5. 5.Max-Planck-Institut für ZellbiologieBBA Institut für Pflanzenschutz im ObstbauDossenheimGermany