Plant Cell Reports

, Volume 23, Issue 5, pp 339–344

Stimulation of asiaticoside accumulation in the whole plant cultures of Centella asiatica (L.) Urban by elicitors

Authors

  • O. T. Kim
    • Department of BiologyChonnam National University
  • M. Y. Kim
    • Department of BiologyChonnam National University
  • M. H. Hong
    • Department of BiologyChonnam National University
  • J. C. Ahn
    • Department of Life SciencesSeonam University
    • Department of BiologyChonnam National University
Physiology and Biochemistry

DOI: 10.1007/s00299-004-0826-7

Cite this article as:
Kim, O.T., Kim, M.Y., Hong, M.H. et al. Plant Cell Rep (2004) 23: 339. doi:10.1007/s00299-004-0826-7

Abstract

The effects of a number of different elicitors on asiaticoside production in whole plant cultures of Centella asiatica were studied, including yeast extract, CdCl2, CuCl2 and methyl jasmonate (MJ). Only MJ and yeast extract stimulated asiaticoside production—1.53 and 1.41-fold, respectively. Maximum asiaticoside production was achieved following treatment with 0.1 mM MJ (116.8 mg/l). The highest asiaticoside production (342.72 mg/l) was obtained after 36 days of elicitation in cultures treated with 0.1 mM MJ and 0.025 mg/l 1-phenyl-3-(1,2,3-thidiazol-5-yl)urea (TDZ). Interestingly, MJ not only stimulated the production of asiaticoside but also had an important role in the senescence of C. asiatica. Although asiaticoside content did not change when TDZ was added to medium containing an elicitor, TDZ did increase shoot growth of C. asiatica. We discuss the interactive roles of MJ and TDZ in secondary metabolic production and biomass in whole plants of C. asiatica

Keywords

AsiaticosideCentella asiaticaWhole plant culturesMethyl jasmonate

Abbreviations

DMSO

Dimethyl sulfoxide

HPLC

High performance liquid chromatography

MJ

Methyl jasmonate

TDZ

1-Phenyl-3-(1,2,3-thidiazol-5-yl)urea

Copyright information

© Springer-Verlag 2004