Plant Cell Reports

, Volume 22, Issue 8, pp 584–593

Antifungal activity of stilbenes in in vitro bioassays and in transgenic Populus expressing a gene encoding pinosylvin synthase

  • S.-K. Seppänen
  • L. Syrjälä
  • K. von Weissenberg
  • T. H. Teeri
  • L. Paajanen
  • A. Pappinen
Genetic Transformation and Hybridization

DOI: 10.1007/s00299-003-0728-0

Cite this article as:
Seppänen, SK., Syrjälä, L., von Weissenberg, K. et al. Plant Cell Rep (2004) 22: 584. doi:10.1007/s00299-003-0728-0

Abstract

The effect of two stilbene compounds, pinosylvin and resveratrol, on the growth of several fungi was evaluated in plate tests. Wood decay tests were carried out with birch and aspen samples impregnated with the two stilbenes. In plate experiments, resveratrol had an enhancing effect on growth at concentrations where pinosylvin was already enough to prevent the growth of most fungi studied. Pinosylvin impregnated at 0.2% (w/w) concentration significantly reduced the decay caused by all fungi except Phellinus tremulae. In contrast, a resveratrol content of 0.8%, did not protect the wood from decay. A pinosylvin-synthase-encoding gene from Pinus sylvestris was transferred into aspen (Populus tremula) and two hybrid aspen clones (Populus tremula×tremuloides) by Agrobacterium tumefaciens-mediated transformation. Transgenic plants accumulated pinosylvin synthase-specific mRNA and showed stilbene synthase enzyme activity in vitro. Transgenic aspen line H4 showed increased resistance to Phellinus tremulae, while two hybrid aspen transformants decayed faster than the control trees. However, we were unable to detect the accumulation of stilbenes in the transgenic plantlets.

Keywords

PinosylvinResveratrolDecay fungiTransformation

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • S.-K. Seppänen
    • 1
  • L. Syrjälä
    • 2
  • K. von Weissenberg
    • 1
  • T. H. Teeri
    • 1
  • L. Paajanen
    • 3
  • A. Pappinen
    • 1
  1. 1.Department of Applied Biology University of HelsinkiHelsinkiFinland
  2. 2. Suonenjoki Research StationFinnish Forest Research InstituteSuonenjokiFinland
  3. 3.VTT Building and TransportFinland