Plant Cell Reports

, Volume 21, Issue 4, pp 365–373

An evaluation of factors affecting the efficiency of Agrobacterium-mediated transformation of Citrus paradisi (Macf.) and production of transgenic plants containing carotenoid biosynthetic genes

  •  M. Costa
  •  W. Otoni
  •  G. Moore
Genetics and Genomics

DOI: 10.1007/s00299-002-0533-1

Cite this article as:
Costa, M., Otoni, W. & Moore, G. Plant Cell Rep (2002) 21: 365. doi:10.1007/s00299-002-0533-1

Abstract.

An improved protocol for Agrobacterium-mediated transformation of Duncan grapefruit (Citrus paradisi Macf.) epicotyl explants was developed by examining the effects of six different factors on the efficiency of transformation and combining the best treatment for each factor. The preculturing of explants and the composition of the cocultivation medium were the factors that most influenced transformation efficiency. The optimized protocol was successfully employed in the production of transgenic grapefruit plants containing the carotenoid biosynthetic genes phytoene synthase, phytoene desaturase, or lycopene-β-cyclase under constitutive expression. With an eventual goal of metabolically engineering grapefruit with multiple genes, hygromycin as a selectable marker and BIBAC as a transformation vector for large pieces of DNA were also tested.

Citrus paradisi Grapefruit Genetic transformation Carotenoids 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  M. Costa
    • 1
  •  W. Otoni
    • 2
  •  G. Moore
    • 1
  1. 1.Horticultural Sciences Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA
  2. 2.Plant Biology Department, Federal University of Viçosa, Viçosa, MG 36571-000, Brazil
  3. 3.Present address: Laboratório de Estudos em Meio Ambiente (LEMA), Centro de Pesquisa e Extensão, Universidade Católica do Salvador, Salvador, BA 40710-000, Brazil

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