Plant Cell Reports

, Volume 20, Issue 12, pp 1135–1140

Tight control of growth and cell differentiation in photoautotrophically growing moss (Physcomitrellapatens) bioreactor cultures

  •  A. Hohe
  •  E. Decker
  •  G. Gorr
  •  G. Schween
  •  R. Reski
Cell Biology and Morphogenesis

DOI: 10.1007/s00299-002-0463-y

Cite this article as:
Hohe, A., Decker, E., Gorr, G. et al. Plant Cell Rep (2002) 20: 1135. doi:10.1007/s00299-002-0463-y

Abstract.

The use of the moss Physcomitrella patens as a production system for heterologous proteins requires highly standardised culture conditions. For this purpose a semi-continuous photoautotrophic bioreactor culture of Physcomitrella was established. This culture grew stably for 7 weeks in a 5-l bioreactor with a dilution rate of 0.22/day. Enrichment of the air for aeration in a batch bioreactor culture with 2% (v/v) CO2 resulted in an increase in the specific growth rate to 0.57/day. Changes in the pH of the semi-continuous bioreactor culture medium between pH 4.5 and pH 7.0 influenced protonema differentiation; however it did not negatively affect the growth rate compared to uncontrolled pH. The advantages of Physcomitrella as a system for the production of heterologous proteins in plants are discussed.

CO2 enrichment pH control Photoautotrophic culture Plant cell suspension Semi-continuous bioreactor culture 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  A. Hohe
    • 1
  •  E. Decker
    • 1
  •  G. Gorr
    • 1
  •  G. Schween
    • 1
  •  R. Reski
    • 1
  1. 1.Freiburg University, Plant Biotechnology, Sonnenstrasse 5, 79104 Freiburg, Germany
  2. 2.Present address: Greenovation Biotech GmbH, Bötzinger Str. 29b, 79111 Freiburg, Germany

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