Genetic Transformation and Hybridization

Plant Cell Reports

, Volume 20, Issue 11, pp 1022-1032

Androgenic dihaploids from somatic hybrids between Solanum melongena and S. aethiopicum group gilo as a source of resistance to Fusarium oxysporum f. sp. melongenae

  •  F. RizzaAffiliated withIstituto Sperimentale per l'Orticoltura, Via Paullese 28, 26836 Montanaso Lombardo (LO), Italy
  • ,  G. MennellaAffiliated withIstituto Sperimentale per l'Orticoltura, Via Cavalleggeri 25, 84098 Pontecagnano (SA), Italy
  • ,  C. CollonnierAffiliated withBât. 360, Morphogénèse Végétale Expérimentale, Université Paris Sud, 91405 Orsay Cedex, France
  • ,  D. SihachakrAffiliated withBât. 360, Morphogénèse Végétale Expérimentale, Université Paris Sud, 91405 Orsay Cedex, France
  • ,  V. KashyapAffiliated withDepartment of Genetics, University of Delhi – South Campus, Benito Juarez Road, New Delhi-21, India
  • ,  M. RajamAffiliated withDepartment of Genetics, University of Delhi – South Campus, Benito Juarez Road, New Delhi-21, India
  • ,  M. PresteràAffiliated withIstituto Sperimentale per l'Orticoltura, Via Paullese 28, 26836 Montanaso Lombardo (LO), Italy
  • ,  G. RotinoAffiliated withIstituto Sperimentale per l'Orticoltura, Via Paullese 28, 26836 Montanaso Lombardo (LO), Italy

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Abstract.

Dihaploid plants were obtained through anther culture of somatic hybrids between eggplant and Solanum aethiopicum gilo. The androgenic origin of the dihaploids was demonstrated by ploidy determination (flow cytometry and chloroplast counting) and isozyme and molecular (I-SSR and RAPDs) analyses. The androgenic plants showed significant morphological variability in the traits analysed. Pollen viability in the diploid androgenic plants was drastically reduced with respect to the somatic hybrids; however most of the dihaploids produced parthenocarpic fruits. S. aethiopicum and the somatic hybrids showed complete resistance to fungal wilt caused by Fusarium oxysporum f. sp. melongenae. Out of the 41 dihaploids inoculated, 34 were symptomless. The population of androgenic plants developed may represent a useful source for introgression of the Fusarium resistance trait into eggplant.

Androgenesis Eggplant Solanum aethiopicum gr. gilo Somatic hybrids Fusarium oxysporum f. sp. melongenae