Original Article

Rheumatology International

, Volume 32, Issue 12, pp 3951-3956

First online:

PAI-1 mRNA expression and plasma level in rheumatoid arthritis: relationship with 4G/5G PAI-1 polymorphism

  • José Francisco Muñoz-ValleAffiliated withDepartamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara Email author 
  • , Sandra Luz Ruiz-QuezadaAffiliated withDepartamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara
  • , Edith Oregón-RomeroAffiliated withDepartamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara
  • , Rosa Elena Navarro-HernándezAffiliated withDepartamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara
  • , Eduardo Castañeda-SaucedoAffiliated withUnidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero
  • , Ulises De la Cruz-MossoAffiliated withUnidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero
  • , Berenice Illades-AguiarAffiliated withUnidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero
  • , Marco Antonio Leyva-VázquezAffiliated withUnidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero
  • , Natividad Castro-AlarcónAffiliated withUnidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero
    • , Isela Parra-RojasAffiliated withUnidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Rheumatoid arthritis (RA) is a chronic inflammatory disease affecting the synovial membrane, cartilage and bone. PAI-1 is a key regulator of the fibrinolytic system through which plasminogen is converted to plasmin. The plasmin activates the matrix metalloproteinase system, which is closely related with the joint damage and bone destruction in RA. The aim of this study was to investigate the relationship between 4G/5G PAI-1 polymorphism with mRNA expression and PAI-1 plasma protein levels in RA patients. 113 RA patients and 123 healthy subjects (HS) were included in the study. The 4G/5G PAI-1 polymorphism was determined by polymerase chain reaction–restriction fragment length polymorphism method; the PAI-1 mRNA expression was determined by real-time PCR; and the soluble PAI-1 (sPAI-1) levels were quantified using an ELISA kit. No significant differences in the genotype and allele frequencies of 4G/5G PAI-1 polymorphism were found between RA patients and HS. However, the 5G/5G genotype was the most frequent in both studied groups: RA (42%) and HS (44%). PAI-1 mRNA expression was slightly increased (0.67 fold) in RA patients with respect to HS (P = 0.0001). In addition, in RA patients, the 4G/4G genotype carriers showed increased PAI-1 mRNA expression (3.82 fold) versus 4G/5G and 5G/5G genotypes (P = 0.0001), whereas the sPAI-1 plasma levels did not show significant differences. Our results indicate that the 4G/5G PAI-1 polymorphism is not a marker of susceptibility in the Western Mexico. However, the 4G/4G genotype is associated with high PAI-1 mRNA expression but not with the sPAI-1 levels in RA patients.

Keywords

Rheumatoid arthritis PAI-1 Polymorphism Messenger RNA