Rheumatology International

, Volume 29, Issue 11, pp 1343–1347

Expression of IL-23 and IL-17 and effect of IL-23 on IL-17 production in ankylosing spondylitis

  • Xinwei Wang
  • Zhiming Lin
  • Qiujing Wei
  • Yingjuan Jiang
  • Jieruo Gu
Original Paper

DOI: 10.1007/s00296-009-0883-x

Cite this article as:
Wang, X., Lin, Z., Wei, Q. et al. Rheumatol Int (2009) 29: 1343. doi:10.1007/s00296-009-0883-x

Abstract

The objective of this study was to investigate the expression of IL-23 and IL-17 and the influence of IL-23 on IL-17 production in ankylosing spondylitis (AS) patients. IL-23 and IL-17 levels in the serum and supernatants of cultured peripheral blood mononuclear cells (PBMCs) were determined by ELISA. IL-23p19 mRNA expression in PBMCs were analyzed using RT-PCR. The patients with AS at active stage showed elevated levels of IL-23 and IL-17 in the serum and supernatants of cultured PBMCs. A higher expression of IL-23p19 mRNA in PBMCs of AS patients was also observed. A significantly enhanced production of IL-17 in the supernatants of cultured PBMCs was found in the presence of recombinant IL-23 and this effect was more significant in patients with AS. The results suggest that IL-23 and IL-17 may play critical roles in the pathogenesis of AS and IL-23-stimulated production of IL-17 by PBMCs may be responsible for the development of AS.

Keywords

Ankylosing spondylitisIL-23IL-17

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Xinwei Wang
    • 1
  • Zhiming Lin
    • 1
  • Qiujing Wei
    • 1
  • Yingjuan Jiang
    • 1
  • Jieruo Gu
    • 1
  1. 1.Department of RheumatologyThird Affiliated Hospital of Sun Yat-Sen UniversityGuangzhouPeople’s Republic of China