Rheumatology International

, Volume 28, Issue 10, pp 979–986

High levels of Lymphotoxin-Beta (LT-Beta) gene expression in rheumatoid arthritis synovium: clinical and cytokine correlations

  • Killian P. O’Rourke
  • G. O’Donoghue
  • C. Adams
  • H. Mulcahy
  • C. Molloy
  • C. Silke
  • M. Molloy
  • F. Shanahan
  • F. O’Gara
Original Article

DOI: 10.1007/s00296-008-0574-z

Cite this article as:
O’Rourke, K.P., O’Donoghue, G., Adams, C. et al. Rheumatol Int (2008) 28: 979. doi:10.1007/s00296-008-0574-z

Abstract

Lymphotoxin-Beta (LT-Beta) is implicated in lymphoid follicle development, production of pro-inflammatory cytokines, and can enhance the proliferation of fibroblasts and synoviocytes. The objective of this study was to investigate LT-Beta and LT-BetaReceptor (LT-BetaR) gene expression in RA patient synovium and blood samples compared with control individuals, and correlate with LT-Alpha and TNF-Alpha gene expression and disease parameters. RT-PCR was used to investigate the gene expression of LT-Beta, LT-BetaR, TNF-Alpha and LT-Alpha in the blood and synovium of RA patients and a control group of individuals. LT-Beta gene expression was significantly higher in RA patient synovium compared to control synovium (P = 0.005). There was a significant positive correlation between LT-Beta and LT-Alpha gene expression in both the synovium (P = 0.001) and blood (P = 0.002) of RA patients. LT-Beta gene expression was significantly higher in RA patient synovial samples that were inflamed to a moderately severe degree compared to those inflamed to a minimal degree (P = 0.02). Analysis of clinical variables revealed a significant positive correlation between LT-BetaR gene expression in RA patient synovium and Pain VAS Score (P = 0.01) and also HAQ Score (P = 0.01). Increased LT-Beta gene expression occurs in RA synovium and correlates with the degree of inflammation. LT-Beta may play a role in RA disease pathogenesis by contributing to a more intense inflammatory reaction in the synovium.

Keywords

Lymphotoxin LT-Beta Rheumatoid Arthritis Synovium 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Killian P. O’Rourke
    • 1
    • 4
  • G. O’Donoghue
    • 1
  • C. Adams
    • 1
  • H. Mulcahy
    • 1
  • C. Molloy
    • 1
  • C. Silke
    • 2
  • M. Molloy
    • 2
  • F. Shanahan
    • 3
  • F. O’Gara
    • 1
  1. 1.Department of Microbiology, Biomerit Research CenterUniversity College CorkCorkIreland
  2. 2.Department of RheumatologyCork Regional HospitalCorkIreland
  3. 3.Department of MedicineCork Regional HospitalCorkIreland
  4. 4.Department of RheumatologyMusgrove Park HospitalTauntonUK