Rheumatology International

, Volume 27, Issue 12, pp 1099–1111

Intra-articular injection of hyaluronate and indomethacin in rabbits with antigen-induced arthritis

  • Yow-Jen Lo
  • Ming-Thau Sheu
  • Wen-Chi Tsai
  • Yun-Ho Lin
  • Jau-Le Li
  • Yu-Chih Liang
  • Chi-Ching Chang
  • Ming-Shium Hsieh
  • Chien-Ho Chen
Original Article

DOI: 10.1007/s00296-007-0346-1

Cite this article as:
Lo, YJ., Sheu, MT., Tsai, WC. et al. Rheumatol Int (2007) 27: 1099. doi:10.1007/s00296-007-0346-1

Abstract

Combined effects of hyaluronate and indomethacin in the treatment of rabbits with antigen-induced arthritis (AIA) were evaluated by assessing joint swelling, C-reactive protein (CRP) and prostaglandin E2 (PGE2) levels with periodic intra-articular (ia) injections of hyaluronate alone (HA group) and with either a low or high concentration of indomethacin (LI-HA or HI-HA group). End-point analyses included matrix metalloproteinases-3 (MMP-3) activity and macroscopic and histological joint examinations. Results demonstrated that treatment in LI-HA and HI-HA groups resulted in statistically significant suppression of CRP, PGE2, and MMP-3 in comparison with those of HA group. Inhibition of serum CRP was only observed in LI-HA group. The order of serum MMP-3 inhibition was LI-HA>HI-HA>HA. Based on macroscopic and histological analyses of pannus formation, hyperplasia, inflammation, joint leakage and erosion, and loss of proteoglycan, the only statistically significant improvement was shown in LI-HA group compared to HA group and HI-HA group compared to control group.

Keywords

Antigen-induced arthritisHyaluronateIndomethacinCRPPGE2MMP

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Yow-Jen Lo
    • 1
  • Ming-Thau Sheu
    • 1
  • Wen-Chi Tsai
    • 2
  • Yun-Ho Lin
    • 3
  • Jau-Le Li
    • 4
  • Yu-Chih Liang
    • 5
  • Chi-Ching Chang
    • 6
  • Ming-Shium Hsieh
    • 4
  • Chien-Ho Chen
    • 5
  1. 1.College of PharmacyTaipei Medical UniversityTaipeiTaiwan, ROC
  2. 2.Department of Orthopedic Surgery Min-Sheng HealthcareTaoyuanTaiwan, ROC
  3. 3.Department of PathologyTaipei Medical UniversityTaipeiTaiwan, ROC
  4. 4.Department of Orthopedics and TraumatologyTaipei Medical University Hospital, Taipei Medical UniversityTaipeiTaiwan, ROC
  5. 5.School of Medical TechnologyTaipei Medical UniversityTaipeiTaiwan, ROC
  6. 6.Department of Internal MedicineTaipei Medical University Hospital, Taipei Medical UniversityTaipeiTaiwan, ROC