Current Genetics

, Volume 40, Issue 3, pp 186–194

Secondary DNA structure analysis of the coding strand switch regions of five Leishmania major Friedlin chromosomes

Authors

  • Valentina Tosato
    • Department of Biology, Genomics Group, University of Trieste, Trieste, 34148, Italy
  • Laura Ciarloni
    • Department of Biology, Genomics Group, University of Trieste, Trieste, 34148, Italy
  • Alasdair C. Ivens
    • The Sanger Centre, Hinxton, Cambridgeshire, CB10 1SA, UK
  • Marie-Adèle Rajandream
    • The Sanger Centre, Hinxton, Cambridgeshire, CB10 1SA, UK
  • Bart G. Barrell
    • The Sanger Centre, Hinxton, Cambridgeshire, CB10 1SA, UK
  • Carlo V. Bruschi
    • Microbiology Group, International Centre for Genetic Engineering and Biotechnology, AREA Science Park, Padriciano 99, 34012 Trieste, Italy
Research Article

DOI: 10.1007/s002940100246

Cite this article as:
Tosato, V., Ciarloni, L., Ivens, A.C. et al. Curr Genet (2001) 40: 186. doi:10.1007/s002940100246

Abstract.

As part of the EULEISH international genome project, a region of 74,674 nucleotides from chromosome 21 of Leishmania major Friedlin was subcloned and sequenced; and 31 new coding sequences were predicted. Of particular interest was a unique coding strand switching region covering 1.6 kb of DNA; and this was subjected to further investigation. Bioinformatic analysis of this region revealed an unusually high AT composition, a lack of putative hairpins and a strong curvature of the DNA in agreement with the structural characteristics of similar regions of other Leishmania chromosomes. These observations and a comparison with the secondary DNA structure of four other Leishmania chromosomes and chromosomes of different organisms could suggest a functional role of this region in transcription and mitotic division.

Leishmania major Friedlin genome Switching point DNA secondary structures Bioinformatic analysis

Copyright information

© Springer-Verlag 2001