Current Genetics

, Volume 36, Issue 6, pp 383–389

Complementary mating types of Mucor circinelloides show electrophoretic karyotype heterogeneity

  • José María Díaz-Mínguez
  • M. Angeles López-Matas
  • Arturo P. Eslava
ORIGINAL PAPER

DOI: 10.1007/s002940050513

Cite this article as:
Díaz-Mínguez, J., López-Matas, M. & Eslava, A. Curr Genet (1999) 36: 383. doi:10.1007/s002940050513

Abstract

Electrophoretic karyotypes of ten strains of Mucor circinelloides f. lusitanicus were generated by contour-clamped homogeneous electric field (CHEF) gel-electrophoresis. Most of the strains analyzed showed polymorphisms, but a different main karyotype pattern could be correlated with each mating type. Genome structure was further analyzed by gene assignment to the chromosome-sized DNAs. Nonradioactive hybridization techniques identified the chromosomal localization of seven cloned genes. The hybridization patterns confirmed the similarity between the mating-type (−) strains and showed some heterogeneity among the mating-type (+) strains. Linkage was found between genes pyrF and chs3 in all the strains, between the gene leuA and the rDNA in all mating-type (−) strains and ATCC 1216b (+), and between chs2 and the rDNA in CBS 969.68 (+). This is the first time that gene linkage in M. circinelloides has been reported, but in some cases the linkage relationships obtained are strain-dependent.

Key words Electrophoretic karyotypeChromosomal polymorphismMating-typeMucor circinelloides

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • José María Díaz-Mínguez
    • 1
  • M. Angeles López-Matas
    • 1
  • Arturo P. Eslava
  1. 1.Area de Genética. Departamento de Microbiología y Genética, Universidad de Salamanca, Avda Campo Charro s/n, E-37007 Salamanca, Spain e-mail: josediaz@gugu.usal.es Tel.: 34-23-294663; Fax: 34-23-294663ES