Current Genetics

, Volume 36, Issue 4, pp 195–200

A ‘natural’ mutation in Saccharomyces cerevisiae strains derived from S288c affects the complex regulatory gene HAP1 (CYP1)

  • M. Gaisne
  • A.-M. Bécam
  • J. Verdière
  • C. J. Herbert
ORIGINAL PAPER

DOI: 10.1007/s002940050490

Cite this article as:
Gaisne, M., Bécam, AM., Verdière, J. et al. Curr Genet (1999) 36: 195. doi:10.1007/s002940050490

Abstract

The HAP1 gene encodes a complex transcriptional regulator of many genes involved in electron-transfer reactions and is essential in anaerobic or heme-depleted conditions. We show here that strains derived from S288c carry a defective Ty1 element inserted in the 3′ region of the HAP1 ORF. This mutant allele acts as a HAP1 null allele in terms of cytochrome c expression and CYC1 UAS1-dependent transcription, but is able to sustain limited growth in heme-depleted conditions.

Key wordsSaccharomyces cerevisiaeHAP1/CYP1S288cTy1

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • M. Gaisne
    • 1
  • A.-M. Bécam
    • 1
  • J. Verdière
    • 1
  • C. J. Herbert
    • 1
  1. 1.Centre de Génétique Moléculaire, Laboratoire propre du CNRS associéà l'Université Pierre et Marie Curie, F-91198, Gif-sur-Yvette, France e-mail: Herbert@CGM.CNRS-GIF.FR Tel.: +33-1-69 82 31 91 Fax: +33-1-69 07 55 39FR