Current Genetics

, Volume 34, Issue 5, pp 375–378

Identification and functional analysis of a Kluyveromyces lactis homologue of the SPT4 gene of Saccharomyces cerevisiae

Authors

  • Imrich Hikkel
    • Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B2, 84215 Bratislava, Slovak Republic e-mail: subik@fns.uniba.sk Tel.: +421-7-60296631 Fax: +421-7-65429064
  • Yvetta Gbelská
    • Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B2, 84215 Bratislava, Slovak Republic e-mail: subik@fns.uniba.sk Tel.: +421-7-60296631 Fax: +421-7-65429064
  • Július Šubík
    • Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B2, 84215 Bratislava, Slovak Republic e-mail: subik@fns.uniba.sk Tel.: +421-7-60296631 Fax: +421-7-65429064
ORIGINAL PAPER

DOI: 10.1007/s002940050409

Cite this article as:
Hikkel, I., Gbelská, Y. & Šubík, J. Curr Genet (1998) 34: 375. doi:10.1007/s002940050409

Abstract

Sequence analysis of a DNA fragment containing the KlCOX18 gene originating from chromosome II of the yeast Kluyveromyces lactis revealed the presence of an adjacent open reading frame (ORF) for a protein exhibiting 78.4% identity with the Saccharomyces cerevisiae Spt4p. Based on the identical length (102 aa) and the conservation of the zinc-finger motif found in Spt4p we named this ORF KlSPT4. When expressed in S. cerevisiae the KlSPT4 gene complemented all spt4 mutant phenotypes. It is proposed that KlSpt4p, like its S. cerevisiae counterpart is a protein involved in the establishment or maintenance of the chromatin structure that influences the expression of many yeast genes.

Key words ChromatinSuppressionYeastTranscription elongation

Copyright information

© Springer-Verlag Berlin Heidelberg 1998