Current Genetics

, Volume 34, Issue 3, pp 183–191

Ace2p, a regulator of CTS1 (chitinase) expression, affects pseudohyphal production in Saccharomyces cerevisiae

  • Lorraine King
  • G. Butler

DOI: 10.1007/s002940050384

Cite this article as:
King, L. & Butler, G. Curr Genet (1998) 34: 183. doi:10.1007/s002940050384


Some diploid strains of Saccharomyces cerevisiae can grow both as a spherical yeast form and as a filamentous pseudohyphal form. Most yeasts capable of forming pseudohyphae possess a functional FLO8 gene. We show that disrupting the ACE2 transcription factor results in the production of pseudohyphae in a flo8-1 background. Disrupting the CTS1 (chitinase) gene also produces pseudohyphal growth in this background, but at a reduced level. Invasion of solid media by haploid and diploid cells is increased in ACE2 disruptions, but the diploids adhere poorly to the agar. Σ1278b-derived strains, which generally produce pseudohyphae, have about 30-fold lower chitinase activity than other strains.

Key words PseudohyphaeChitinAce2Swi5Cts1

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Lorraine King
    • 1
  • G. Butler
    • 1
  1. 1.Department of Biochemistry, University College Dublin, Belfield, Dublin 4, Ireland e-mail: Tel.: +353-1-7061583 Fax: +353-1-2837211IE