ORIGINAL PAPER

Current Genetics

, Volume 34, Issue 3, pp 183-191

First online:

Ace2p, a regulator of CTS1 (chitinase) expression, affects pseudohyphal production in Saccharomyces cerevisiae

  • Lorraine KingAffiliated withDepartment of Biochemistry, University College Dublin, Belfield, Dublin 4, Ireland e-mail: gbutler@ucd.ie Tel.: +353-1-7061583 Fax: +353-1-2837211
  • , G. ButlerAffiliated withDepartment of Biochemistry, University College Dublin, Belfield, Dublin 4, Ireland e-mail: gbutler@ucd.ie Tel.: +353-1-7061583 Fax: +353-1-2837211

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Abstract

Some diploid strains of Saccharomyces cerevisiae can grow both as a spherical yeast form and as a filamentous pseudohyphal form. Most yeasts capable of forming pseudohyphae possess a functional FLO8 gene. We show that disrupting the ACE2 transcription factor results in the production of pseudohyphae in a flo8-1 background. Disrupting the CTS1 (chitinase) gene also produces pseudohyphal growth in this background, but at a reduced level. Invasion of solid media by haploid and diploid cells is increased in ACE2 disruptions, but the diploids adhere poorly to the agar. Σ1278b-derived strains, which generally produce pseudohyphae, have about 30-fold lower chitinase activity than other strains.

Key words Pseudohyphae Chitin Ace2 Swi5 Cts1