Current Genetics

, Volume 33, Issue 1, pp 21–28

Over-expression of the yeast BFR2 gene partially suppresses the growth defects induced by Brefeldin A and by four ER-to-Golgi mutations

  • Sandrine Chabane
  • Emmanuel Gachet
  • François Képès
ORIGINAL PAPER

DOI: 10.1007/s002940050304

Cite this article as:
Chabane, S., Gachet, E. & Képès, F. Curr Genet (1998) 33: 21. doi:10.1007/s002940050304

Abstract

The fungal metabolite Brefeldin A (BFA) disrupts the Golgi apparatus and its incoming protein flux. We developed a genetic approach to identify yeast proteins involved in the protein transport step that BFA blocks. The BFR2 gene (YDR299W) was thus isolated as a high-copy suppressor of the growth defects induced by BFA in a sensitive strain of Saccharomyces cerevisiae. Although BFR2 over-expression did not cause a secretory block or slow-down, it partially suppressed the growth defect of four mutants blocked at the step of budding or docking of small vesicles en route to the Golgi (sec13-1, sec16-2, sec23-1, ypt1-1). The essential BFR2 gene was predicted to encode an extremely hydrophilic product containing two short regions with potential coiled-coils, one of which corresponds to a cluster of acidic residues.

Key wordsSaccharomyces cerevisiae Protein transport Golgi apparatus Brefeldin A 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Sandrine Chabane
    • 1
  • Emmanuel Gachet
    • 1
  • François Képès
    • 1
  1. 1.Service de Biochimie et de Génétique Moléculaire, DBCM/DSV, Bât. 142, CEA/Saclay, F-91191 Gif cedex, France Fax: (33) 1 69 08 47 12 e-mail: kepes@jonas.saclay.cea.frFR

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