Current Genetics

, Volume 32, Issue 3, pp 203–208

Variability of karyotypes and RAPD types in genetically related strains of Cryptococcus neoformans

  • T. Boekhout
  • Alex van Belkum
ORIGINAL PAPER

DOI: 10.1007/s002940050267

Cite this article as:
Boekhout, T. & van Belkum, A. Curr Genet (1997) 32: 203. doi:10.1007/s002940050267

Abstract

Variation in karyotypes and RAPD patterns of genetically related strains of Cryptococcus neoformans were analyzed. Capsular and filamentous mutants usually differ in their karyotypes from wild-types, but the RAPD patterns were found to be similar. Karyotype differences were observed in most heterothallic matings, but RAPD patterns remained identical. After self-sporulation of a diploid strain, minor chromosomal length polymorphism and minor changes in the RAPD types occurred. Three mechanisms, either alone or in combination, may in varying degrees contribute to the karyotype variation of C. neoformans: (1) mitotically induced changes; (2) karyotype changes as a result of meiotic recombination, and (3) mutagen-induced changes. The present data do not support the meiotic maintenance hypothesis, which claims that the amount of CLP generated is inversely proportional to the frequency of meiosis.

Key wordsCryptococcus neoformansKaryotypeRAPD patternsMatingMutant

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • T. Boekhout
    • 1
  • Alex van Belkum
    • 2
  1. 1.Yeast Division, Centraalbureau voor Schimmelcultures, Julianalaan 67, 2628 BC Delft, The NetherlandsNL
  2. 2.Department of Bacteriology, University Hospital Rotterdam, Rotterdam, The NetherlandsNL