ORIGINAL PAPER

Current Genetics

, Volume 32, Issue 1, pp 66-72

First online:

Transfer of an autonomously replicating vector between vegetatively incompatible biotypes of Colletotrichum gloeosporioides

  • Agnieszka M. PoplawskiAffiliated withCooperative Research Centre for Tropical Plant Pathology, The John Hines Building, The University of Queensland, Brisbane 4072, Australia
  • , Chaozu HeAffiliated withCooperative Research Centre for Tropical Plant Pathology, The John Hines Building, The University of Queensland, Brisbane 4072, Australia
  • , John A. G. IrwinAffiliated withCooperative Research Centre for Tropical Plant Pathology, The John Hines Building, The University of Queensland, Brisbane 4072, Australia
  • , J. M. MannersAffiliated withCooperative Research Centre for Tropical Plant Pathology, The John Hines Building, The University of Queensland, Brisbane 4072, Australia

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Abstract

Previous research has indicated that biotypes A and B of Colletotrichum gloeosporioides that infect Stylosanthes spp. in Australia are asexual and vegetatively incompatible. Selectable marker genes conferring resistance either to hygromycin or phleomycin were introduced into isolates of these biotypes. Vectors conferring resistance to hygromycin and carrying telomeric sequences from Fusarium oxysporum replicated autonomously in C. gloeosporioides and gave frequencies of transformation 100-times higher than vectors that integrated into the genome. Monoconidial colonies resistant to both antibiotics were recovered when hygromycin-resistant biotype-A transformants carrying an autonomously replicating vector were paired in culture with a phleomycin-resistant biotype-B transformant carrying integrative vector sequences. Molecular analysis of double antibiotic-resistant progeny indicated that they contained the autonomous vector in a biotype-B genetic background. Results indicate that transfer of the autonomous vector had occurred from biotype A to biotype B, demonstrating the potential for transfer of genetic information between these biotypes.

Key wordsStylosanthes Horizontal transfer Anthracnose Vegetative compatibility