Current Genetics

, Volume 31, Issue 5, pp 401–407

A mutation in cytochrome oxidase subunit 2 restores respiration of the mutant pet ts1402

Authors

  • Wolfram Meyer
    • Institut für Allgemeine Botanik, Universität Hamburg, Ohnhorststrasse 18, D-22609 Hamburg, Germany
  • Mathias Bauer
    • Institut für Allgemeine Botanik, Universität Hamburg, Ohnhorststrasse 18, D-22609 Hamburg, Germany
  • E. Pratje
    • Institut für Allgemeine Botanik, Universität Hamburg, Ohnhorststrasse 18, D-22609 Hamburg, Germany
ORIGINAL PAPER

DOI: 10.1007/s002940050222

Cite this article as:
Meyer, W., Bauer, M. & Pratje, E. Curr Genet (1997) 31: 401. doi:10.1007/s002940050222
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Abstract

The yeast PET1402/OXA1 gene encoding a 44.8-kDa protein is required for mitochondrial biogenesis. Substitution of Leu240 to serine in the protein results in an accumulation of the precursor form of the mitochondrially encoded subunit 2 of cytochrome oxidase (Cox2) and temperature-sensitive respiration. This temperature sensitivity can be suppressed by a mutation in the cox2 gene changing Ala189 of the Cox2 protein to proline. In the cox2-ts1402 double mutant respiration is restored without removal of the Cox2 pre-sequence. The suppression suggests an interaction of the Pet1402 protein with the cytochrome oxidase complex. Antibodies raised against the predicted C-terminus and the tagged N-terminus of the Pet1402 protein reacted with a 37-kDa polypeptide. This protein, present in the mitochondrial fraction, is localized within the inner membrane. The difference in size can be explained by the removal of the predicted mitochondrial-targeting sequence from the Pet1402 protein. The mitochondrial localization of the protein points to a direct interaction with the cytochrome oxidase complex.

Key words Cytochrome oxidaseMitochondrial localizationPET1402/OXA1Saccharomyces cerevisiae
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© Springer-Verlag Berlin Heidelberg 1997