Current Genetics

, Volume 31, Issue 3, pp 272–275

Transformation of the Oomycete Saprolegnia monoïca to hygromycin-B resistance

  • M. Mort-Bontemps
  • Michel Fèvre
ORIGINAL PAPER

DOI: 10.1007/s002940050205

Cite this article as:
Mort-Bontemps, M. & Fèvre, M. Curr Genet (1997) 31: 272. doi:10.1007/s002940050205

Abstract

Saprolegnia monoïca was stably transformed to hygromycin resistance using the plasmids pTH210 and pHAM34H containing a bacterial phosphotransferase gene fused to regulatory sequences from genes of another Oomycete. Vectors pBT6, pCM54, used to transform higher fungi, yielded no stable transformants. DNA hybridizations indicated that transformation resulted from a single-copy integration of the transforming vector. Development of non-resistant subcultures from transformed colonies revealed that the transgene could become silent and was not uniformly expressed in the transformants.

Key words Transformation Oomycete Saprolegnia monoïca Hygromycin 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • M. Mort-Bontemps
    • 1
  • Michel Fèvre
    • 1
  1. 1.Laboratoire de Biologie Cellulaire Fongique, CGMC UMR CNRS 5534, Université Lyon 1, 43, Boulevard 11 Novembre 1918, F-69622 Villeurbanne Cedex, FranceFR