Current Genetics

, Volume 31, Issue 3, pp 241–247

STP1, a gene involved in pre-tRNA processing in yeast, is important for amino-acid uptake and transcription of the permease gene BAP2

  • Marianne U. Jørgensen
  • Claes Gjermansen
  • Helge A. Andersen
  • Morten C. Kielland-Brandt
ORIGINAL PAPER

DOI: 10.1007/s002940050201

Cite this article as:
Jørgensen, M., Gjermansen, C., Andersen, H. et al. Curr Genet (1997) 31: 241. doi:10.1007/s002940050201

Abstract

The bap1 mutant of Saccharomyces cerevisiae was previously isolated by its reduced uptake of branched-chain amino acids. In the present study, the corresponding wild-type gene was cloned and partial sequencing and subsequent genetic analysis revealed identity to STP1, a gene involved in tRNA maturation. The decrease in amino-acid uptake caused by stp1 mutations is independent of GCN4. It was previously found that the BAP2 promoter can be activated by the presence of amino acids, notably leucine, in the medium. We found that this activation depends on STP1. As a simple hypothesis we propose that Stp1p is a transcription factor which activates BAP2, and probably other amino-acid permease genes.

Key wordsSaccharomyces cerevisiae  Amino-acid uptake  STP1  BAP2  GCN4

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Marianne U. Jørgensen
    • 1
  • Claes Gjermansen
    • 1
  • Helge A. Andersen
    • 1
  • Morten C. Kielland-Brandt
    • 1
  1. 1.Department of Yeast Genetics, Carlsberg Laboratory Gamle Carlsberg Vej 10, DK-2500 Copenhagen Valby, DenmarkDK