Current Genetics

, Volume 30, Issue 4, pp 332–337

Meiotic segregation and recombination of the intergenic spacer of the ribosomal DNA in the ectomycorrhizal basidiomycete Laccaria bicolor

  • Marc-Andreé Selosse
  • G. Costa
  • Céline Di Battista
  • François Le Tacon
  • F. Martin
ORIGINAL PAPER

DOI: 10.1007/s002940050141

Cite this article as:
Selosse, M., Costa, G., Battista, C. et al. Curr Genet (1996) 30: 332. doi:10.1007/s002940050141

Abstract

 The aim of this study was to clarify the inheritance of the nuclear ribosomal DNA (rDNA) in the ectomycorrhizal basidiomycete Laccaria bicolor S238N in order to resolve inter- and within-strain relationships in forest ecosystems. PCR amplification of the intergenic spacer (IGS) was carried out in the dikaryotic mycelium and its haploid progeny. In the dikaryotic mycelium, multiple amplification products were produced for the 25s/5s (IGS1) and 5s/17s (IGS2) intergenic spacers. The 4.5- and 4.0-kb fragments of IGS2 (haplotypes α and β, respectively) were observed to occur in a 1:1 ratio within the haploid progeny as a result of divergent IGS haplotypes in the two separate nuclei. Recombinant monokaryons having both types of IGS2 occurred at a low frequency (6.5%; 60 kb per centimorgan) during meiosis. Haplotypes α and β of IGS1 cross-hybridized forming heteroduplexes during the PCR temperature cycle. The two IGS1 haplotypes differed only by the repeat number of a TA2C3 motif and co-segregated with the IGS2 haplotypes. Heteroduplex formation and IGS polymorphism provide information that is helpful in distinguishing between introduced exotic L. bicolor S238N and indigenous populations of Laccaria spp. in forest ecosystems.

Key words Laccaria bicolorBasidiomyceteIntergenic spacersRibosomal DNAMeiotic recombinationHeteroduplex

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Marc-Andreé Selosse
    • 1
  • G. Costa
    • 1
  • Céline Di Battista
    • 1
  • François Le Tacon
    • 1
  • F. Martin
    • 1
  1. 1.Equipe de Microbiologie Forestière, Centre de Recherche de Nancy, Institut National de la Recherche Agronomique, F-54280 Champenoux, FranceFR