Current Genetics

, Volume 30, Issue 2, pp 101–106

Differential intrachromosomal hyper-recombination phenotype of spt4 and spt6 mutants of S. cerevisiae

  • Francisco Malagón
  • Andrés Aguilera

DOI: 10.1007/s002940050107

Cite this article as:
Malagón, F. & Aguilera, A. Curr Genet (1996) 30: 101. doi:10.1007/s002940050107


In order to test whether mutations affecting transcription also affect DNA recombination, we have determined the influence of a number of snf/swi and spt/sin transcriptional regulatory mutations on the recombination of a DNA inverted repeat. Among the nine different mutations analyzed, we found that spt4 and spt6 confer a significant hyper-recombination phenotpye. Both mutations produced increases in the frequencies of reciprocal exchange/gene conversion and deletion events ranging from 1- to 15-fold above the wild-type levels, as determined in six direct repeat systems and one inverted repeat. The frequency of mitotic recombination between homologs, determined at one chromosomal locus, was not affected. We discuss the intrachromosomal hyper-recombination phenotype of spt4 an spt6 on the basis of the possible functions of SPT4 and SPT6 on transcriptional regulation and on chromatin structure.

Key words  Transcription DNA repeats Hyper-recombination SWI/SNF SPT/SIN 
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© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Francisco Malagón
    • 1
  • Andrés Aguilera
    • 1
  1. 1.Departamento de Genética, Facultad de Biología, Universidad de Sevilla, E-41012 Sevilla, SpainES

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