Current Genetics

, Volume 38, Issue 1, pp 17–22

Isolation and characterization of KlUBP2, a ubiquitin hydrolase gene of Kluyveromyces lactis that can suppress a ts-mutation in CBF2, a gene encoding a centromeric protein of Saccharomyces cerevisiae

Authors

  • A. A. Winkler
    • Institute for Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands e-mail: Steensma@rulbim.leidenuniv.nl Tel.: 31-71-527 4947; Fax: 31-71-527 4999
  • R. Korstanje
    • Institute for Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands e-mail: Steensma@rulbim.leidenuniv.nl Tel.: 31-71-527 4947; Fax: 31-71-527 4999
  • B. J. M. Zonneveld
    • Institute for Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands e-mail: Steensma@rulbim.leidenuniv.nl Tel.: 31-71-527 4947; Fax: 31-71-527 4999
  • P. J. J. Hooykaas
    • Institute for Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands e-mail: Steensma@rulbim.leidenuniv.nl Tel.: 31-71-527 4947; Fax: 31-71-527 4999
  • H. Y. Steensma
    • Institute for Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands e-mail: Steensma@rulbim.leidenuniv.nl Tel.: 31-71-527 4947; Fax: 31-71-527 4999
ORIGINAL PAPER

DOI: 10.1007/s002940000129

Cite this article as:
Winkler, A., Korstanje, R., Zonneveld, B. et al. Curr Genet (2000) 38: 17. doi:10.1007/s002940000129

Abstract

 The Kluyveromyces lactis UBP2 gene was isolated as a suppressor of a temperature-sensitive mutation in CBF2, a gene coding for a centromere-binding protein of Saccharomyces cerevisiae. The UBP genes are hydrolases than can cleave a ubiquitin moiety from a protein substrate. KlUBP2 is not essential for growth since a disruption of the KlUBP2 gene had little effect, except for a slight decrease in the growth rate. The stability of centromere-containing plasmids was not influenced either. In addition to KlUBP2, five S. cerevisiae genes involved in the ubiquitination pathway could suppress the ts-mutation in the CBF2 gene, namely UBA1, UBA2, UBP1, UBP2 and YUH1, although YUH1 was the only one that could do this like KlUBP2 from a single-copy plasmid. Surprisingly, these genes encode proteins with antagonistic activity as two, UBA1 and UBA2, are ubiquitin-activating enzymes whereas the other three are de-ubiquitinating hydrolases.

Key words Kluyveromyces lactisCBF2UBP2Ubiquitination

Copyright information

© Springer-Verlag Berlin Heidelberg 2000