, Volume 38, Issue 1, pp 1-7

Absence of F1-ATPase activity in Kluyveromyces lactis lacking the ɛ subunit

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Abstract

The mitochondrial F1-ATPase is a multimeric enzyme, comprised of 3α, 3β, γ, δ and ɛ subunits, that is primarily responsible for the synthesis of ATP in eukaryotic cells. Recent work has shown that the F1 complex of the petite-negative yeast Kluyveromyces lactis, with specific mutations in the α, β or γ subunits, has a novel function that suppresses lethality caused by loss of mtDNA. Previously, genes for the four largest subunits of K. lactis F1 have been identified and characterised. In this study the gene coding for the ɛ-subunit of F1, KlATPɛ, has been isolated and found to encode a polypeptide of 61 amino acids with only 32 residues identical to those in the protein from Saccharomyces cerevisiae. Strains carrying a null mutation of KlATPɛ are respiratory deficient while the introduction of ATPɛ from S. cerevisiae restores growth on non-fermentable carbon sources. In contrast to S. cerevisiae, K. lactis disrupted in ATPɛ does not have a detectable F1-related mitochondrial ATP hydrolysis activity, suggesting that the ɛ-subunit plays a critical role in the formation of the catalytic sector of F1. With a disrupted KlATPɛ, the ρo-lethality suppressor function of F1 carrying the atp2-1 and atp1-6 alleles is abolished. However, inactivation of the ɛ subunit does not eliminate the ρo-viable phenotype of the atp1-1, atp2-9, atp3-2 mutants. It is suggested that the absence of ɛ may effect the assembly or stability of F1 in the wild-type, atp 2-1 and atp1-6 strains, whereas the defect can be suppressed by the atp1-1, atp2-9 and atp3-2 mutations in the α, β and γ subunits respectively.

Received: 9 November 1999 / 11 February 2000