Research Article

Current Genetics

, Volume 58, Issue 3, pp 165-177

Genome-wide analysis of cell wall-related genes in Tuber melanosporum

  • Raffaella BalestriniAffiliated withIstituto per la Protezione delle Piante del CNR, UOS TorinoDipartimento di Biologia Vegetale, UniTO Email author 
  • , Fabiano SilloAffiliated withIstituto per la Protezione delle Piante del CNR, UOS TorinoDipartimento di Biologia Vegetale, UniTO
  • , Annegret KohlerAffiliated withUMR 1136, INRA-Nancy Université, Interactions Arbres/Microorganismes
  • , Georg SchneiderAffiliated withBioinformatics Institute, A*Star
  • , Antonella FaccioAffiliated withIstituto per la Protezione delle Piante del CNR, UOS TorinoDipartimento di Biologia Vegetale, UniTO
  • , Emilie TisserantAffiliated withUMR 1136, INRA-Nancy Université, Interactions Arbres/Microorganismes
  • , Francis MartinAffiliated withUMR 1136, INRA-Nancy Université, Interactions Arbres/Microorganismes
  • , Paola BonfanteAffiliated withIstituto per la Protezione delle Piante del CNR, UOS TorinoDipartimento di Biologia Vegetale, UniTO

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Abstract

A genome-wide inventory of proteins involved in cell wall synthesis and remodeling has been obtained by taking advantage of the recently released genome sequence of the ectomycorrhizal Tuber melanosporum black truffle. Genes that encode cell wall biosynthetic enzymes, enzymes involved in cell wall polysaccharide synthesis or modification, GPI-anchored proteins and other cell wall proteins were identified in the black truffle genome. As a second step, array data were validated and the symbiotic stage was chosen as the main focus. Quantitative RT-PCR experiments were performed on 29 selected genes to verify their expression during ectomycorrhizal formation. The results confirmed the array data, and this suggests that cell wall-related genes are required for morphogenetic transition from mycelium growth to the ectomycorrhizal branched hyphae. Labeling experiments were also performed on T. melanosporum mycelium and ectomycorrhizae to localize cell wall components.

Keywords

Tuber melanosporum Fungal genome Cell wall Ectomycorrhizae Symbiotic interactions