Current Genetics

, Volume 58, Issue 1, pp 35–47

[NSI+] determinant has a pleiotropic phenotypic manifestation that is modulated by SUP35, SUP45, and VTS1 genes

  • Anton A. Nizhnikov
  • Zalina M. Magomedova
  • Alexandr A. Rubel
  • Alexandra M. Kondrashkina
  • Sergey G. Inge-Vechtomov
  • Alexey P. Galkin
Research Article

DOI: 10.1007/s00294-011-0363-1

Cite this article as:
Nizhnikov, A.A., Magomedova, Z.M., Rubel, A.A. et al. Curr Genet (2012) 58: 35. doi:10.1007/s00294-011-0363-1

Abstract

We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI+] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI+] possesses yeast prion features and does not correspond to previously identified yeast prion determinants. Here, we show that [NSI+] enhances nonsense codon read-through and inhibits vegetative growth in S. cerevisiae. Using a large-scale overexpression screen to identify genes that impact the phenotypic effects of [NSI+], we found that the SUP35 and SUP45 genes encoding the translation termination factors eRF3 and eRF1, respectively, modulate nonsense suppression in [NSI+] strains. The VTS1 gene encodes an NQ-enriched RNA-binding protein that enhances nonsense suppression in [NSI+] and [nsi] strains. We demonstrate that VTS1 overexpression, like [NSI+] induction, causes translational read-through and growth defects in S. cerevisiae.

Keywords

Yeast[NSI+]PrionSUP35SUP45VTS1

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Anton A. Nizhnikov
    • 1
    • 2
  • Zalina M. Magomedova
    • 1
    • 2
  • Alexandr A. Rubel
    • 1
    • 2
  • Alexandra M. Kondrashkina
    • 2
  • Sergey G. Inge-Vechtomov
    • 1
    • 2
  • Alexey P. Galkin
    • 1
    • 2
  1. 1.St. Petersburg Branch Vavilov Institute of General Genetics (Russian Academy of Science)St. PetersburgRussia
  2. 2.Department of Genetics and BreedingSt. Petersburg State UniversitySt. PetersburgRussia