[NSI+] determinant has a pleiotropic phenotypic manifestation that is modulated by SUP35, SUP45, and VTS1 genes
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- Nizhnikov, A.A., Magomedova, Z.M., Rubel, A.A. et al. Curr Genet (2012) 58: 35. doi:10.1007/s00294-011-0363-1
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We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI+] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI+] possesses yeast prion features and does not correspond to previously identified yeast prion determinants. Here, we show that [NSI+] enhances nonsense codon read-through and inhibits vegetative growth in S. cerevisiae. Using a large-scale overexpression screen to identify genes that impact the phenotypic effects of [NSI+], we found that the SUP35 and SUP45 genes encoding the translation termination factors eRF3 and eRF1, respectively, modulate nonsense suppression in [NSI+] strains. The VTS1 gene encodes an NQ-enriched RNA-binding protein that enhances nonsense suppression in [NSI+] and [nsi−] strains. We demonstrate that VTS1 overexpression, like [NSI+] induction, causes translational read-through and growth defects in S. cerevisiae.