Current Genetics

, Volume 56, Issue 5, pp 467–478

[NSI+]: a novel non-Mendelian nonsense suppressor determinant in Saccharomyces cerevisiae

  • Alsu F. Saifitdinova
  • Anton A. Nizhnikov
  • Artem G. Lada
  • Alexandr A. Rubel
  • Zalina M. Magomedova
  • Valentina V. Ignatova
  • Sergey G. Inge-Vechtomov
  • Alexey P. Galkin
Research Article

DOI: 10.1007/s00294-010-0314-2

Cite this article as:
Saifitdinova, A.F., Nizhnikov, A.A., Lada, A.G. et al. Curr Genet (2010) 56: 467. doi:10.1007/s00294-010-0314-2

Abstract

Non-Mendelian determinants that control heritable traits in yeast are subdivided into two major groups—one that includes DNA- or RNA-based elements and another that comprises protein-based factors that are analogous to mammalian prion. All yeast non-Mendelian determinants show dominant inheritance, and some of them demonstrate cytoplasmic infectivity. Only prions, however, harbor-specific features, such as high frequency of induction following overproduction of prion-encoding protein, loss of the protein’s normal function, and reversible curability. Here, we describe a novel nonchromosomal determinant that, in addition to [PSI+] and [ISP+], is involved in epigenetic control of nonsense suppression. This determinant, which we have designated [NSI+], causes nonsense suppression in the strains bearing the N-terminal-deleted or -modified SUP35 gene, but has no manifestation in the strains with the intact copy of SUP35. [NSI+] shows dominant non-Mendelian inheritance, reversible curability and may be transmitted by cytoduction, albeit with low frequency. Similar to yeast prions, this determinant can be cured by deletion or mutational inactivation of Hsp104. We have shown that [NSI+] does not correspond to the already identified yeast prions. Based on the data obtained, we hypothesize that [NSI+] is a novel prion factor involved in epigenetic control of nonsense suppression.

Keywords

YeastNon-Mendelian determinantPrionNonsense suppressionSup35

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Alsu F. Saifitdinova
    • 1
    • 2
  • Anton A. Nizhnikov
    • 2
  • Artem G. Lada
    • 2
    • 3
  • Alexandr A. Rubel
    • 1
    • 2
  • Zalina M. Magomedova
    • 1
  • Valentina V. Ignatova
    • 2
  • Sergey G. Inge-Vechtomov
    • 1
    • 2
  • Alexey P. Galkin
    • 1
    • 2
  1. 1.St. Petersburg Branch, Vavilov Institute of General GeneticsRussian Academy of ScienceSt. PetersburgRussia
  2. 2.Department of Genetics and BreedingSt. Petersburg State UniversitySt. PetersburgRussia
  3. 3.Eppley Institute for Research in Cancer, 986805 Nebraska Medical CenterOmahaUSA