Current Genetics

, Volume 55, Issue 4, pp 365–380

Comprehensive and quantitative analysis of yeast deletion mutants defective in apical and isotropic bud growth

  • Machika Watanabe
  • Daisuke Watanabe
  • Satoru Nogami
  • Shinichi Morishita
  • Yoshikazu Ohya
Research Article

DOI: 10.1007/s00294-009-0251-0

Cite this article as:
Watanabe, M., Watanabe, D., Nogami, S. et al. Curr Genet (2009) 55: 365. doi:10.1007/s00294-009-0251-0

Abstract

To obtain a comprehensive understanding of the budding phase transition, 4,711 Saccharomyces cerevisiae haploid nonessential gene deletion mutants were screened with the image processing program CalMorph, and 35 mutants with a round bud and 173 mutants with an elongated bud were statistically identified. We classified round and elongated bud mutants based on factors thought to affect the duration of the apical bud growth phase. Two round bud mutants (arc18 and sac6) were found to be defective in apical actin patch localization. Several elongated bud mutants demonstrated a delay of cell cycle progression at the apical growth phase, suggesting that these mutants have a defect in the control of cell cycle progression.

Keywords

Cell morphologyApical growthPolarized growthActinYeast

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Machika Watanabe
    • 1
  • Daisuke Watanabe
    • 1
  • Satoru Nogami
    • 1
  • Shinichi Morishita
    • 2
  • Yoshikazu Ohya
    • 1
  1. 1.Department of Integrated Biosciences, Graduate School of Frontier SciencesUniversity of TokyoChibaJapan
  2. 2.Department of Computational Biology, Graduate School of Frontier SciencesUniversity of TokyoChibaJapan