Current Genetics

, Volume 52, Issue 1, pp 11–22

Mating-type loci of heterothallic Diaporthe spp.: homologous genes are present in opposite mating-types

Research Article

DOI: 10.1007/s00294-007-0132-3

Cite this article as:
Kanematsu, S., Adachi, Y. & Ito, T. Curr Genet (2007) 52: 11. doi:10.1007/s00294-007-0132-3

Abstract

Sexual reproduction of fungi is governed by genes located on the mating-type (MAT) locus. To analyze the MAT locus of the genus Diaporthe (anamorph: Phomopsis), a large genera within the ascomycetous class Sordariomycetes, we cloned and sequenced loci MAT1-1 and MAT1-2 from two heterothallic Diaporthe species, designated as Diaporthe W- and G-types (four isolates in total). The mating-type loci structures of Diaporthe W- and G-types were similar; MAT1-1 isolates had a MAT locus containing three genes, MAT1-1-1, MAT1-1-2 and MAT1-1-3, as was the case with other Sordariomycetes, and in contrast to other Sordariomycetes, MAT1-2 isolates had genes homologous to MAT1-1-2 and MAT1-1-3, in addition to MAT1-2-1. Expression analysis by RT-PCR revealed that all the mating-type genes of Diaporthe W-type were transcriptionally active during vegetative growth. The structure of MAT loci of Diaporthe W- and G-types is distinct from that in other heterothallic filamentous ascomycetes, which have dissimilar gene structure in opposite mating-type loci. This unique structure is informative to discussing the evolutionary history and function of mating-type genes of Sordariomycete fungi.

Keywords

PyrenomycetesMating-type gene organizationEvolution

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Satoko Kanematsu
    • 1
  • Yoshihiko Adachi
    • 2
  • Tsutae Ito
    • 1
  1. 1.Apple Research StationNational Institute of Fruit Tree Science, NAROMoriokaJapan
  2. 2.National Agricultural Research Center for Tohoku Region, NAROMoriokaJapan