Current Genetics

, Volume 51, Issue 3, pp 171–186

InterB multigenic family, a gene repertoire associated with subterminal chromosome regions of Encephalitozoon cuniculi and conserved in several human-infecting microsporidian species

Authors

  • Ndongo Dia
    • Equipe Parasitologie Moléculaire et Cellulaire, Laboratoire de Biologie des ProtistesUMR CNRS 6023, Bâtiment Biologie A, Université Blaise Pascal
  • Laurence Lavie
    • Equipe Parasitologie Moléculaire et Cellulaire, Laboratoire de Biologie des ProtistesUMR CNRS 6023, Bâtiment Biologie A, Université Blaise Pascal
  • Guy Méténier
    • Equipe Parasitologie Moléculaire et Cellulaire, Laboratoire de Biologie des ProtistesUMR CNRS 6023, Bâtiment Biologie A, Université Blaise Pascal
  • Bhen S. Toguebaye
    • Laboratoire de Parasitologie Générale, Département de Biologie Animale, Faculté des Sciences et TechnologiesUniversité Cheikh Anta Diop
  • Christian P. Vivarès
    • Equipe Parasitologie Moléculaire et Cellulaire, Laboratoire de Biologie des ProtistesUMR CNRS 6023, Bâtiment Biologie A, Université Blaise Pascal
    • Equipe Parasitologie Moléculaire et Cellulaire, Laboratoire de Biologie des ProtistesUMR CNRS 6023, Bâtiment Biologie A, Université Blaise Pascal
Research Article

DOI: 10.1007/s00294-006-0114-x

Cite this article as:
Dia, N., Lavie, L., Méténier, G. et al. Curr Genet (2007) 51: 171. doi:10.1007/s00294-006-0114-x

Abstract

Microsporidia are fungi-related obligate intracellular parasites that infect numerous animals, including man. Encephalitozoon cuniculi harbours a very small genome (2.9 Mbp) with about 2,000 coding sequences (CDSs). Most repeated CDSs are of unknown function and are distributed in subterminal regions that mark the transitions between subtelomeric rDNA units and chromosome cores. A potential multigenic family (interB) encoding proteins within a size range of 579–641 aa was investigated by PCR and RT-PCR. Thirty members were finally assigned to the E. cuniculi interB family and a predominant interB transcript was found to originate from a newly identified gene on chromosome III. Microsporidian species from eight different genera infecting insects, fishes or mammals, were tested for a possible intra-phylum conservation of interB genes. Only representatives of the Encephalitozoon, Vittaforma and Brachiola genera, differing in host range but all able to invade humans, were positive. Molecular karyotyping of Brachiola algerae showed a complex set of chromosome bands, providing a haploid genome size estimate of 15–20 Mbp. In spite of this large difference in genome complexity, B. algerae and E. cuniculi shared some similar interB gene copies and a common location of interB genes in near-rDNA subterminal regions.

Keywords

Encephalitozoon cuniculiBrachiola algeraeChromosome extremityMultigenic familyKARD 2D-PFGE

Copyright information

© Springer-Verlag 2007