Current Genetics

, Volume 47, Issue 6, pp 389–399

Heterogeneity of intron presence or absence in rDNA genes of the lichen species Physcia aipolia and P. stellaris

  • Dawn M. Simon
  • Cora L. Hummel
  • Sara L. Sheeley
  • Debashish Bhattacharya
Research Article

DOI: 10.1007/s00294-005-0581-5

Cite this article as:
Simon, D.M., Hummel, C.L., Sheeley, S.L. et al. Curr Genet (2005) 47: 389. doi:10.1007/s00294-005-0581-5
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Abstract

Intron origin and evolution are of high interest, yet the rates of insertion and loss are unclear. To investigate their spread, we studied ribosomal (r)DNA introns from the closely related lichens Physcia aipolia and P. stellaris. Both taxa are replete with rDNA spliceosomal introns and autocatalytic group I introns, many of which show presence/absence polymorphism when screened with the PCR approach. This initially suggested that Physcia could be a model for studying intron retention and loss. However, during the course of a population-level analysis, we discovered widespread intron presence/absence heterogeneity within lichen thalli. To address this result, we sequenced multiple clones encoding nuclear rDNA and the single-copy elongation factor-1α (EF-1α) from individual thalli. These data showed extensive rDNA heterogeneity within individuals, rather than the presence of multiple fungi within a thallus. Our results suggest that considerable care must be taken when interpreting intron presence/absence in lichen rDNA, an observation that has general implications for the study of rDNA intron evolution.

Keywords

Group I intron Intron loss Lichen Physcia rDNA Spliceosomal intron 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Dawn M. Simon
    • 1
  • Cora L. Hummel
    • 1
  • Sara L. Sheeley
    • 1
  • Debashish Bhattacharya
    • 1
  1. 1.Department of Biological Sciences and the Roy J. Carver Center for Comparative GenomicsUniversity of IowaIowa CityUSA
  2. 2.Department of Biological SciencesUniversity of CalgaryCalgaryCanada

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