Current Genetics

, Volume 47, Issue 2, pp 67–83

Enhanced mitochondrial degradation of yeast cytochrome c with amphipathic structures

  • Xi Chen
  • Richard P. Moerschell
  • David A. Pearce
  • Durga D. Ramanan
  • Fred Sherman
Research Article

DOI: 10.1007/s00294-004-0552-2

Cite this article as:
Chen, X., Moerschell, R.P., Pearce, D.A. et al. Curr Genet (2005) 47: 67. doi:10.1007/s00294-004-0552-2

Abstract

The dispensable N-terminus of iso-1-cytochrome c (iso-1) in the yeast Saccharomyces cerevisiae was replaced by 11 different amphipathic structures. Rapid degradation of the corresponding iso-1 occurred, with the degree of degradation increasing with the amphipathic moments; and this amphipathic-dependent degradation was designated ADD. ADD occurred with the holo-forms in the mitochondria but not as the apo-forms in the cytosol. The extreme mutant type degraded with a half-life of approximately 12 min, whereas the normal iso-1 was stable over hours. ADD was influenced by the ρ+ state and by numerous chromosomal genes. Most importantly, ADD appeared to be specifically suppressed to various extents by deletions of any of the YME1, AFG3, or RCA1 genes encoding membrane-associated mitochondrial proteases, probably because the amphipathic structures caused a stronger association with the mitochondrial inner membrane and its associated proteases. The use of ADD assisted in the differentiation of substrates of different mitochondrial degradation pathways.

Keywords

Saccharomyces cerevisiaeCytochrome cMitochondrial AAA proteasesMitochondrial proteasesAmphipathic structuresMitochondrial degradation

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Xi Chen
    • 1
  • Richard P. Moerschell
    • 2
  • David A. Pearce
    • 1
  • Durga D. Ramanan
    • 1
  • Fred Sherman
    • 1
  1. 1.Department of Biochemistry and Biophysics, School of Medicine and DentistryUniversity of RochesterRochesterUSA
  2. 2.Life Sciences GroupBio-RadHerculesUSA