Current Genetics

, Volume 44, Issue 4, pp 184–194

Saccharomyces cerevisiae Ats1p interacts with Nap1p, a cytoplasmic protein that controls bud morphogenesis

  • Christina M. Shields
  • Rachel Taylor
  • Tara Nazarenus
  • Joseph Cheatle
  • Ann Hou
  • Audrey Tapprich
  • Alexis Haifley
  • Audrey L. Atkin
Research Article

DOI: 10.1007/s00294-003-0442-z

Cite this article as:
Shields, C.M., Taylor, R., Nazarenus, T. et al. Curr Genet (2003) 44: 184. doi:10.1007/s00294-003-0442-z

Abstract

Saccharomyces cerevisiae ATS1 (α-tubulin suppressor 1) was originally identified as a high-copy suppressor of class two α-tubulin mutations and was proposed to have a regulatory role in coordinating the microtubule state with the cell cycle. Here, we show that Ats1p interacts with Nap1p, a cytoplasmic protein that regulates the activity of the Cdc28p/Clb2p complex. Loss of Nap1p results in a delayed switch from polar to isotropic bud growth. The delayed switch results in elongated buds. Nap1p and Ats1p interact in two-hybrid and co-immunoprecipitation assays. Both nap1Δ and ats1Δ cells have a Clb2p-dependent elongated bud morphology. Deletion of ATS1 partially suppresses the elongated bud morphology and benomyl resistance of nap1Δ mutants. Our results suggest Ats1p might regulate coordination of the microtubule state with the cell cycle through an interaction with Nap1p.

Keywords

Ats1p Nap1p Clb2p Cell cycle Morphogenesis checkpoint 

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Christina M. Shields
    • 1
  • Rachel Taylor
    • 1
  • Tara Nazarenus
    • 1
  • Joseph Cheatle
    • 1
  • Ann Hou
    • 1
  • Audrey Tapprich
    • 1
  • Alexis Haifley
    • 1
  • Audrey L. Atkin
    • 1
  1. 1.School of Biological SciencesUniversity of Nebraska—LincolnLincolnUSA

Personalised recommendations