Research Article

Current Genetics

, Volume 44, Issue 3, pp 138-147

First online:

Cryptosporidium parvum Cpn60 targets a relict organelle

  • Christina E. RiordanAffiliated withWadsworth Center, New York State Department of Health, Axelrod Institute for Public HealthDepartment of Biomedical Sciences, SUNY Albany School of Public Health
  • , Jeffrey G. AultAffiliated withWadsworth Center, New York State Department of Health, Axelrod Institute for Public Health
  • , Susan G. LangrethAffiliated withDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences
  • , Janet S. KeithlyAffiliated withWadsworth Center, New York State Department of Health, Axelrod Institute for Public HealthDepartment of Biomedical Sciences, SUNY Albany School of Public Health Email author 

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Abstract

Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites (CpCpn60) was analyzed and antibodies were generated for localization of the peptide. Sequence and phylogenetic analyses indicated that CpCpn60 is a mitochondrial isotype and that antibodies against it localize to the rough endoplasmic reticulum-enveloped remnant organelle of C. parvum sporozoites. These data show this organelle is of mitochondrial origin.

Keywords

Apicomplexan Relict mitochondrion Chaperonin 60 (Cpn60)