Current Genetics

, Volume 42, Issue 2, pp 94–102

The mitochondrial genome can be altered or lost without lethal effect in the petite-negative yeast Debaryomyces (Schwanniomyces) occidentalis

  • Caroline S. Fernet
  • Desmond G. Clark-Walker
  • Maurice L. Claisse
Research Article

DOI: 10.1007/s00294-002-0337-4

Cite this article as:
Fernet, C.S., Clark-Walker, D.G. & Claisse, M.L. Curr Genet (2002) 42: 94. doi:10.1007/s00294-002-0337-4

Abstract.

The nature of mutations affecting several cytochrome-deficient mutants of Debaryomyces (Schwanniomyces) occidentalis has been characterized. The DR12 mutant, which is deficient in cytochrome b, and the B10Mn mutant, which is deficient in cytochromes b and a, a3, are deleted in the mitochondrial CYB and COX1 genes respectively. The B10 strain, which is partially deficient in cytochrome b, has no detectable change in its mitochondrial DNA and possibly carries nuclear lesion(s). These three mutants, unlike the rho and rho° "petite" mutants of Saccharomyces cerevisiae, can still grow on non-fermentable substrates, due to the development of a salicylhydroxamic acid (SHAM)-sensitive alternative pathway linked to phosphorylation at site 1. A gly mutant lacking mtDNA and respiratory capacity has been isolated. For the first time, it is demonstrated that mtDNA can be altered or even lost without lethal consequence in D. occidentalis, although this yeast was classified as a petite-negative species.

Debaryomyces (Schwanniomyces) occidentalis Petite-negative Mitochondrial DNA

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Caroline S. Fernet
    • 1
  • Desmond G. Clark-Walker
    • 2
  • Maurice L. Claisse
    • 1
  1. 1.Centre de Génétique Moléculaire, UPR 2167 du CNRS associée a l'UPMC, 1 Avenue de la terrasse, Bât. 26, 91198 Gif-sur-Yvette, France
  2. 2.Molecular Genetics and Evolution Group, Research School of Biological Sciences, Australian National University, P.O. Box 475, Canberra City, ACT 2601, Australia