Polymer Bulletin

, Volume 70, Issue 2, pp 549–561

An improved method for preparing glutaraldehyde cross-linked chitosan–poly(vinyl alcohol) microparticles

Original Paper

DOI: 10.1007/s00289-012-0853-4

Cite this article as:
Campos, E., Coimbra, P. & Gil, M.H. Polym. Bull. (2013) 70: 549. doi:10.1007/s00289-012-0853-4


The features of microparticles, as size, surface structure, and morphology, depend, mainly, on the methodology used for their preparation. Emulsion polymerization techniques are undoubtedly among the most widespread. However, the use of toxic, volatile organic solvents represents a major disadvantage, namely, because of environmental issues. In this study, we prepared glutaraldehyde cross-linked chitosan–poly(vinyl alcohol) microparticles by an improved water-in-oil emulsion technique using corn oil as organic phase. The application of this polymeric blend as microparticle is scarcely investigated. As resulting of the procedure here presented, spherical and smooth surface microparticles were obtained, with mean diameter of 16 μm. The cross-linking reaction between the aldehyde and the amino or the hydroxyl groups formed either an imine (Schiff’s base) or an acetal bond, respectively, as analyzed by infrared spectroscopy. The microparticles here described did not present cytotoxic potential. Accordingly, this study can find promising and successful application in biotechnology.


Microparticle Chitosan Poly(vinyl alcohol) Glutaraldehyde Water-in-oil emulsion Cytotoxicity 

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Chemical Process Engineering and Forest Products Research Centre (CIEPQPF), Department of Chemical Engineering, Faculty of Sciences and TechnologyUniversity of CoimbraCoimbraPortugal
  2. 2.Instituto de Tecnologia Química e BiológicaUniversidade Nova de LisboaOeirasPortugal

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