Current Microbiology

, Volume 40, Issue 4, pp 217–220

Occurrence of Bacillus thuringiensis in Fresh Waters of Japan

  • Tokio  Ichimatsu
  • Eiichi  Mizuki
  • Katsumi  Nishimura
  • Tetsuyuki  Akao
  • Hiroyuki  Saitoh
  • Kazuhiko  Higuchi
  • Michio  Ohba

DOI: 10.1007/s002849910044

Cite this article as:
Ichimatsu, T., Mizuki, E., Nishimura, K. et al. Curr Microbiol (2000) 40: 217. doi:10.1007/s002849910044

Abstract.

Bacillus thuringiensis was recovered at a relatively high frequency from both running and still fresh waters in natural environments of Kyushu, Japan. Of 107 water samples examined, 53 (49.5%) contained this organism. The frequency of B. thuringiensis colonies was 4.4% among 4414 colonies of the Bacillus cereus/B. thuringiensis group. The density of this bacterium in fresh waters averaged 0.45 cfu/ml. Serologically, B. thuringiensis isolates were assigned to 26 H serotypes. Of these, H14/36 (H serovar israelensis/malaysiensis) was the predominant, followed by the serotypes H3abc (kurstaki), H27 (mexicanensis), H3ad (sumiyoshiensis), and H35 (seoulensis). Of 195 isolates, 52 (26.7%) exhibited larvicidal activity against aquatic Diptera; 21 killed Culex pipiens molestus (Culicidae) only, and 31 were active on both the culicine mosquito and the moth-fly, Clogmia albipunctata (Psychodidae). The Diptera-toxic isolates produced spherical or irregularly pointed parasporal inclusions.

Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • Tokio  Ichimatsu
    • 1
  • Eiichi  Mizuki
    • 1
  • Katsumi  Nishimura
    • 1
  • Tetsuyuki  Akao
    • 1
  • Hiroyuki  Saitoh
    • 1
  • Kazuhiko  Higuchi
    • 1
  • Michio  Ohba
    • 2
  1. 1.Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa, Kurume, Fukuoka 839-0861, Japan JP
  2. 2.Institute of Biological Control, Kyushu University, Fukuoka 812-8581, Japan JP