Current Microbiology

, Volume 40, Issue 1, pp 40–46

Molecular Identification of Potentially Probiotic Lactobacilli

  • Denis  Roy
  • Pierre  Ward
  • Daniel  Vincent
  • Francine  Mondou

DOI: 10.1007/s002849910008

Cite this article as:
Roy, D., Ward, P., Vincent, D. et al. Curr Microbiol (2000) 40: 40. doi:10.1007/s002849910008

Abstract.

The rRNA-targeted oligonucleotide probes are useful for the identification of Lactobacillus acidophilus, L. gasseri, L. johnsonii, L. crispatus, and L. amylovorus. However, the oligonucleotide probe designed for L. helveticus hybridized with nucleic acids of type strains of L. gallinarum and L. helveticus. Hence, the similarity among the 73 strains of lactobacilli was evaluated on the basis of their randomly amplified polymorphic DNA (RAPD) profiles derived from five single-primer reactions. These strains were grouped into seven clusters at a similarity level of 30%, which corresponded to six separate species of the L. acidophilus complex (L. johnsonii, L. gallinarum, L. amylovorus, L. crispatus, L. acidophilus, and L. gasseri, respectively) and L. helveticus. For the first time, strains of L. gallinarum were characterized by RAPD and PFGE analyses. The genome length in that species was estimated to be near 1.45 Mb with the summation of ApaI fragments, and near 1.95 Mb with the summation of SmaI fragments.

Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • Denis  Roy
    • 1
  • Pierre  Ward
    • 1
  • Daniel  Vincent
    • 1
  • Francine  Mondou
    • 2
  1. 1.Food Research and Development Centre, Agriculture and Agrifood Canada, 3600 Casavant Blvd. West, Saint-Hyacinthe, Québec, Canada, J2S 8E3 CA
  2. 2.Rosell Institute Inc., Montréal, Québec, Canada CA