Current Microbiology

, Volume 39, Issue 5, pp 302–305

Repeated Ubiquitin Genes in Trichophyton mentagrophytes

  • Rui  Kano
  • Yuka  Nakamura
  • Shinichi  Watanabe
  • Atsuhiko  Hasegawa

DOI: 10.1007/s002849900463

Cite this article as:
Kano, R., Nakamura, Y., Watanabe, S. et al. Curr Microbiol (1999) 39: 302. doi:10.1007/s002849900463
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Abstract.

Using Trichophyton mentagrophytes cDNA as a template, we performed PCR amplification with the UB1S and UB1R primers for isolation of a partial fragment of T. mentagrophytes ubiquitin gene. We screened the T. mentagrophytes cDNA library prepared with Uni-ZAP phage vector by hybridization with the T. mentagrophytes ubiquitin fragment, UB1S-UB1R. The nucleotide and deduced amino acid sequences of T. mentagrophytes ubiquitin gene encoded two ubiquitin repeats in 162 bp to 624 bp. The deduced amino acid sequences of ubiquitin from T. mentagrophytes shared about 98% similarity with those of Saccharomyces cerevisiae, Neurospora crassa, and Cryptococcus neoformans.

Copyright information

© Springer-Verlag New York Inc. 1999

Authors and Affiliations

  • Rui  Kano
    • 1
  • Yuka  Nakamura
    • 2
  • Shinichi  Watanabe
    • 2
  • Atsuhiko  Hasegawa
    • 1
  1. 1.Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866, Kameino, Fujisawa Kanagawa, 252-8501, Japan JP
  2. 2.Department of Dermatology, Teikyo University School of Medicine, Japan JP

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