Current Microbiology

, Volume 35 , Issue 3 , pp 175 –179

Glucose and Galactose Transport in Bifidobacterium bifidum DSM 20082

  • Frédéric  Krzewinski
  • Colette  Brassart
  • Françoise  Gavini
  • Stéphane  Bouquelet

DOI: 10.1007/s002849900234

Cite this article as:
Krzewinski, F., Brassart, C., Gavini, F. et al. Curr Microbiol (1997) 35: 175. doi:10.1007/s002849900234

Abstract.

Sugar uptake was measured with 3H-galactose and 14C-glucose. Galactose transport system was not modified by inhibitors of known translocases and did not present a saturation kinetic with high concentration of galactose. Glucose incorporation was inhibited by lasalocid (cation symport inhibitor) and increased by KCl. The kinetic parameters KM and Vmax were respectively 9.16 mM and 26.56 nmol/min/mg cell protein. On the basis of this study, galactose crossed through the membrane by diffusion, and glucose was incorporated by a cation symport which is regulated by K+ ions.

Copyright information

© Springer-Verlag New York Inc. 1997

Authors and Affiliations

  • Frédéric  Krzewinski
    • 1
  • Colette  Brassart
    • 1
  • Françoise  Gavini
    • 2
  • Stéphane  Bouquelet
    • 1
  1. 1.Laboratoire de Chimie Biologique, UMR No. 111 CNRS, Université des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq cedex, France FR
  2. 2.Institut National de la Recherche Agronomique, Département Nutrition, Alimentation et Sécurité Alimentaire, Unité d'Ecologie et de Physiologie du Système Digestif, Domaine du C.E.R.T.I.A., 59650 Villeneuve d'Ascq cedex, France FR