Current Microbiology

, Volume 35 , Issue 2 , pp 109 –115

Isolation and Characterization of Vibrio carchariae, a Causative Agent of Gastroenteritis in the Groupers, Epinephelus coioides

  • Kah-Ching  Yii
  • Tun-I  Yang
  • Kuo-Kau  Lee

DOI: 10.1007/s002849900221

Cite this article as:
Yii, KC., Yang, TI. & Lee, KK. Curr Microbiol (1997) 35: 109. doi:10.1007/s002849900221

Abstract.

An outbreak of serious mortality among the cultured groupers Epinephelus coioides, characterized by a swollen intestine containing yellow fluid, occurred in the summer of 1993 in Taiwan. A motile strain EmI82KL was isolated from the intestinal yellow fluid of the moribund groupers with tryptic soy agar supplemented with 2% NaCl and/or thiosulfate citrate bile salt sucrose agar. This strain was characterized and identified as Vibrio carchariae and was susceptible to chloramphenicol, doxycycline-HCl, nalidixic acid, oxolinic acid, oxytetracycline, and sulfonamide while resistant to ampicillin and penicillin G. In addition, the strain was neither auto-agglutinating nor hemagglutinating, but it was hemolytic against erythrocytes from sheep, rabbit, tilapia, and grouper. The bacteria could be reisolated from kidney, liver, and the transparent yellow fluid of swollen intestine of moribund groupers after bacterial challenge and re-identified as the same species. The LD50 value was 2.53 × 107 colony forming units/g grouper body weight.

Copyright information

© Springer-Verlag New York Inc. 1997

Authors and Affiliations

  • Kah-Ching  Yii
    • 1
  • Tun-I  Yang
    • 1
  • Kuo-Kau  Lee
    • 1
  1. 1.Department of Aquaculture, National Taiwan Ocean University, 2, Pei-Ning Road, Keelung, Taiwan 202, ROC TW